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1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an observation of the result) 2.Explain the difference in results for the PCR and the RT-PCR from the CD4+ T cells (ie. propose a conclusion)

1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an observation of the result) 2.Explain the difference in results for the PCR and the RT-PCR from the CD4+ T cells (ie. propose a conclusion)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an
observation of the result)
2.Explain the difference in results for the PCR and the RT-PCR from the CD4+ T cells (ie. propose a
conclusion)

Here is a second human gene, called cxx3. The structure indicates the following: the start and end of transcription, the start codon and the stop codon, the location of the two introns (marked with black boxes). The white boxes indicate the binding locations of two PCR primers. PCR CD4+ T cells transcriptional start site RT-PCR CD4+ T cells +1 RT-PCR CD8+ T cells AIG Size markers The researchers carry out a FACS (Fluorescence Activated Cell Sorting) separation of human blood which isolates a relatively pure sample of CD4+ T cells, and a relatively pure sample of CD8+ T cells, both different types of lymphocytes. They carry out a PCR, and a semi-quantitative RT-PCR, on these cell samples. The results are shown on the gel. wells +. intron DNA migration V intron transcriptional stop site Į TAA
Transcribed Image Text:Here is a second human gene, called cxx3. The structure indicates the following: the start and end of transcription, the start codon and the stop codon, the location of the two introns (marked with black boxes). The white boxes indicate the binding locations of two PCR primers. PCR CD4+ T cells transcriptional start site RT-PCR CD4+ T cells +1 RT-PCR CD8+ T cells AIG Size markers The researchers carry out a FACS (Fluorescence Activated Cell Sorting) separation of human blood which isolates a relatively pure sample of CD4+ T cells, and a relatively pure sample of CD8+ T cells, both different types of lymphocytes. They carry out a PCR, and a semi-quantitative RT-PCR, on these cell samples. The results are shown on the gel. wells +. intron DNA migration V intron transcriptional stop site Į TAA
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