Identify the incorrect statements O Taq polymerase can incorporate nucleotides at a rate of -60bp per second at 70°C It is possible to re-amplify PCR products to obtain a higher yield of the target Steps in PCR are typically repeated (cycled) 25-35 times The maximum PCR product length that Taq polymerase can typically amplify is approximately 10 kbp V Extension is the second step in PCR

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: Single stranded DNA fragment of M13 phage DNA shown was mixed with the following  primers P1: AGTAG…

A: Polymerase chain reaction also abbreviated as PCR.A specific DNA fragment is quickly multiplied…

Q: Due to degradation of DNA during food processing what is the best PCR target size for detecting…

A: Food processing conditions such as high temperature, pH variations, enzymatic activities,…

Q: The reason for using Taq polymerase for PCR is that: a. it is heat stable and can withstand the…

A: PCR is a process for synthesising artificial genes using dNTPs, primers, Taq DNA polymerase, and…

Q: Question 35. Theoretically, the PCR process is a true chain reaction but in reality, this is not the…

A: PCR stands for Polymerase Chain Reaction. It is a laboratory technique used to amplify a small…

Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

A:

Q: ased on the knowledge you gained from the cloning module, which of the lanes in the urs is…

A: Agarose gel electrophoresis is used to separate DNA molecules based on their size and analyze DNA…

Q: The synthesis of phosphocreatine is unfavorable. If dur- ng extended periods of rest, e.g., sleeping…

A: Phosphocreatine (PCr) -  It is a phosphorylated creatine molecule that works as transferable reserve…

Q: Method for using PCR to amplify unknown sequences using known sequences by circularizing the…

A: The polymerase chain reaction is an amplification process which generates thousands to millions of…

Q: Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of…

A: Multiplex ligation-dependent probe amplification (MLPA) is an advanced multiplexing technique that…

Q: Agarose gel Can someone help me to understand how to estimate the size of my plasmid cut (pla cut)…

A: The procedure of inserting recombinant DNA into a vector, or a carrier of DNA molecules, to…

Q: Why uncut and cut PCR at different position in the agarose gel? which one can be decided accuracy…

A: *Gel electrophoresis a technique to separate DNA fragments based on their size. *DNA samples are…

Q: 1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA…

A: The gel electrophoresis is routinely used technique in molecular biology laboratories that helps in…

Q: Design forward and reverse primers to amplify this ENTIRE locus by PCR. Design each primer to be 10…

A: The forward and reverse primers are both important components used in PCR for amplifying a target…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: Calculate the annealing temperature (Tm in °C) for the PCR primer below. Do not include units when…

A: Answer. Polymerase chain reaction (PCR), the annealing temperature is the temperature during which…

Q: Following method is used to study long-range chromosome interactions Digestion by…

A: Chromosome interaction refers to the physical interactions and spatial organization of chromosomes…

Q: If your template DNA contains one copy of chromosome 16 with the Alu insertion at PV92, and one copy…

A: PV92 is a human-specific  Alu insertion on chromosome 16.The genetic system of PV92 has only two…

Q: Identify the incorrect statements O Each primer should have 40-60% GC content The annealing…

A: The success of PCR depends on various factors which involves- 1. Template DNA 2. Primers 3. dNTPs 4.…

Q: is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on…

A: Restriction digestion is the fragmentation(cutting) of DNA on very specific sites (restriction…

Q: Synthesis of hybrid genes can occu Directed mutagenesis O Using overlap primers O RT-PCR

A: PCR technique of amplification of a given molecule of DNA using taq polymerase enzyme.

Q: MOLECULAR CLONING • Molecular cloning is the laboratory process used to create recombinant DNA. • It…

A: Recombinant DNA: it is a method of combining two distinct DNA (molecule) sequences, which may or may…

Q: You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using…

A: Bacteria produce the restriction enzyme, a protein that cleaves DNA at certain locations.  The…