(a) To illustrate how the ADP phosphorylative capacity was measured, the diagram below onthe left shows a ³1P NMR spectrum of the arm of a control female subject at rest, and the diagram onthe right the changes measured in the creatine and phosphocreatine levels of the arm at rest andexercise.PCr10 Exer-ciseExer→→→Rest◄cise RestPCr/Pi 9PCr152010Time(min)Isokinetic ergometer exercise of the control femalesubject showing intervals of rest and exercise. Toobtain this graph, a ³1P NMR spectrum was col-lected every 2 min to show the changes in the PCrand Pi content of the arm skeletal muscle tissue.NH₂duringactivity+ATP +C=NH2duringrecoveryYATPPiGATPBATPSP150101520255ppm31P NMR spectrum of the arm of a control sub-ject at rest showing characteristic resonancefeatures for the phosphate residues of ATP,phosphocreatine (PCr), and Pi. At rest thePCr/Pi ratio is ordinarily ~ 8-9.The synthesis of phosphocreatine is unfavorable. If dur-ing extended periods of rest, e.g., sleeping at night,what must be the ADP/ATP ratio if the resting level ofPCr/Cr is 30/1?0™-0-P=0N-H+C=NH₂2+ ADPCH3-NCH₂COO™PhosphocreatineCH3-NCH ₂COO™Creatine

Phosphocreatine (PCr) - It is a phosphorylated creatine molecule that works as transferable reserve…

Answered: The synthesis of phosphocreatine is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

What is the formula used to calculate the number of DNA molecules which will be created for a given number of PCR cycles.
Why is taq polymerase used in pcr reactions.
The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. e. What is the probability that any random male in USA would share the same genotype as the murderer (the match probability)? Assume that all 11 DNA samples analyzed in the diagram are together representative of the USA population as a whole. Show what numbers you would multiply to do this calculation.
How does RT-PCR differ from traditional PCR?
What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)
The ability to multiplex the PCR reactions used in STR analysis (many PCR amplifications occurringin the same tube at the same time) simplifies the DNA profiling technique a great deal. However,the analysis of the PCR products involves measuring only their size and their color, and this is allthe software needs to know in order to figure out which PCR products are from which locus. Howcan the analysis software tell all the various loci apart?
Why is a thermostable form of DNA polymerase (e.g., Taqpolymerase) used in PCR? Is it necessary to use a thermostableform of DNA polymerase in the dideoxy method or in site-directedmutagenesis?
Why would you look for ARGs in a farming context And why specifically in the waste manure produced at a farm? please prehrase and find the answer in the article
(b) The treatment given to the patient was 10 mg of menadione (formula on the right) every 6 hr with a dose of 1 g of ascorbate (Vitamin C) every 6 hr. The rationale for this treatment was that menadione (also called Vitamin K3) could accept electrons from reduced CoQ of the electron transport chain, shuttle them to reduce ascorbate, which, in turn, could reduce cytochrome c(Fe3*) to cytochrome c(Fe2*) that in turn becomes the substrate of Complex IV or cytochrome c oxidase. This artificial sys- tem would bypass the defective Complex III site in the electron transfer chain. To show the effect of this regimen with respect to the ADP phos- phorylative capacity of her skeletal muscle, corresponding 31P NMR spectra are shown on the right. „CH3 2-methyl-1,4-naphthoquinone (menadione)
QUESTION 5: Should PCR primers be complementary to each other? Explain the reasoning for your answer.
Experiment 1: An RT-PCR was run on human cells growing in tissue culture under standard conditions, using the primers described above. A normal PCR was also run on the cells, using the same primers. They were run on an agarose gel shown on the left in the figure (gel 1), in the wells indicated by ‘RT-PCR’ and ‘PCR’. The arrow shows the direction of current in the gel. a) Observe the gel and describe the results for the RT-PCR and PCR (no explanation, just describe). b) Explain why the bands have travelled different distances on the gel.
If a researcher attempted to do a PCR amplification of the slc24a5 gene in the gol1 mutatant line, would their attempts have been successful? Why or why not? No; PCR amplification will not be successful because the gene is deleted in the golb1 mutatant line. No; PCR amplification will not be successful because there is a point mutation in the coding region of the gene. Yes; this mutant line had a point mutation that would not have impacted PCR. Yes; although the gene switch was mutated, it did not impact the promotor sequence. O O O O

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS

The synthesis of phosphocreatine is unfavorable. If dur- ng extended periods of rest, e.g., sleeping at night, what must be the ADP/ATP ratio if the resting level of PCr/Cr is 30/1?