Single stranded DNA fragment of M13 phage DNA shown was mixed with the following primers P1: AGTAG AATTGATGCC ACCTT; P2: TGT CTG GAA GTT TCA TTC CA and incubated in a thermal cycler in the presence of the four dNTPs and Taq DNA polymerase under the PCR conditions. AATGCTACTA CTATTAGTAG AATTGATGCC ACCTTTTCAG CTCGCGCCCC ATAGCTAAAC AGGTTATTGA CCATTTGCGA AATGTATCTA ATGGTCAAAC CGTTCGCAGA ATTGGGAATC AACTGTTACA TGGAATGAAA CTTCCAGACA What is the length of the PCR product? What is the final concentration of PCR product after 10 cycles if the starting template concentration was 1 nM?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Single stranded DNA fragment of M13 phage DNA shown was mixed with the following
primers P1: AGTAG AATTGATGCC ACCTT; P2: TGT CTG GAA GTT TCA TTC CA and incubated in a
thermal cycler in the presence of the four dNTPs and Taq DNA polymerase under the PCR
conditions.
AATGCTACTA CTATTAGTAG AATTGATGCC ACCTTTTCAG CTCGCGCCCC ATAGCTAAAC AGGTTATTGA
CCATTTGCGA AATGTATCTA ATGGTCAAAC CGTTCGCAGA ATTGGGAATC AACTGTTACA TGGAATGAAA
CTTCCAGACA
What is the length of the PCR product?
What is the final concentration of PCR product after 10 cycles if the starting template
concentration was 1 nM?
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