Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost. PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp. How many bands and of what size would you expect to see in individuals who: i) has sickle-cell anaemia (has two βS alleles) ii) carry the disease (sickle cell trait) (has one βS and one βA allele) a. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 55 Sickle cell trait) (has one βS and one βA allele) 1 110 b. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 2 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110 c. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 1 110 d. Individual Number of bands Size of bands (bp) Sickle-cell anaemia (has two βS alleles) 1 110 Sickle cell trait) (has one βS and one βA allele) 2 55 & 110

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βAallele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost.
PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp.

How many bands and of what size would you expect to see in individuals who:

i) has sickle-cell anaemia (has two βSalleles)

ii) carry the disease (sickle cell trait) (has one βS and one βA allele)

Individual	Number of bands	Size of bands (bp)
Sickle-cell anaemia (has two βSalleles)	2	55
Sickle cell trait) (has one βS and one βA allele)	1	110

Individual	Number of bands	Size of bands (bp)
Sickle-cell anaemia (has two βSalleles)	2	110
Sickle cell trait) (has one βS and one βA allele)	2	55 & 110

Individual	Number of bands	Size of bands (bp)
Sickle-cell anaemia (has two βSalleles)	1	110
Sickle cell trait) (has one βS and one βA allele)	1	110

Individual	Number of bands	Size of bands (bp)
Sickle-cell anaemia (has two βSalleles)	1	110
Sickle cell trait) (has one βS and one βA allele)	2	55 & 110

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