Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes, where YES means will ligate, and NO means will not ligate. Restriction enzyme Sphl Nlalll Ncol Nspl Pfl2311 Recognition site 5'-GCATG/C-3' 3'-C/GTACG-5' 5'-CATG/-3' 3'-/GTAC-5' 5'-C/CATGG-3' 3'-GGTAC/C-5' 5'-ACATG/T-3' 3'-T/GTACA-5' 5'-C/GTACG-3' 3'-GCATG/C-5' Compatible with Sphl? YES NO YES NO YES
Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes, where YES means will ligate, and NO means will not ligate. Restriction enzyme Sphl Nlalll Ncol Nspl Pfl2311 Recognition site 5'-GCATG/C-3' 3'-C/GTACG-5' 5'-CATG/-3' 3'-/GTAC-5' 5'-C/CATGG-3' 3'-GGTAC/C-5' 5'-ACATG/T-3' 3'-T/GTACA-5' 5'-C/GTACG-3' 3'-GCATG/C-5' Compatible with Sphl? YES NO YES NO YES
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme
cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA
digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests
of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which
have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes,
where YES means will ligate, and NO means will not ligate.
Restriction
enzyme
Sphl
Nlalll
Ncol
Nspl
Pfl2311
Recognition site
5'-GCATG/C-3'
3'-C/GTACG-5'
5'-CATG/-3'
3'-/GTAC-5'
5'-C/CATGG-3'
3'-GGTAC/C-5'
5'-ACATG/T-3'
3'-T/GTACA-5'
5'-C/GTACG-3'
3'-GCATG/C-5'
Compatible with Sphl?
YES
NO
YES
NO
YES
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