A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: • nutrient broth plus ampicillin • nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which media would allow growth of bacteria that did not take up any plasmids? the nutrient broth and the ampicillin broth the nutrient broth and the tetracycline broth the nutrient broth only the ampicillin broth and the tetracycline broth

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Q: For the Escherichia coli lactose operon genotypes listed, + indicates a functional component, -…

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A:

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Q: Explain how the lac operon can be used to express a recombinant protein in an E. coli cell.

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Q: ORIGIN 1 aagcttctcc accagcgctt tcagctgcgc cagggtctgc tgccggtcct ccgcggcggc 61 gacaggccag gccgactggc…

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Q: 1. What are the basic requirements for the insertion of λ into the E. Coli genome?  2. What special…

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Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.