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position of the Start and Stop. The GFP and RFP genes shown do not have start

position of the Start and Stop. The GFP and RFP genes shown do not have start

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Cre-Lox Reporter Gene System for Genetic Engineering** The Cre-Lox system is an advanced technique used for site-specific DNA modification in genetic engineering. This technology is particularly valuable for its precision in gene expression studies and the manipulation of genetic material. The following explanation provides an overview of a reporter gene construct set up to test the Cre-Lox system functionality within cells. **Construct Diagram Explanation:** - **CMV-Pro:** The construct starts with a Cytomegalovirus (CMV) promoter, a commonly used promoter in genetic engineering due to its high expression levels in mammalian cells. - **LoxP Sites:** Flanking the green fluorescent protein (GFP) gene are the LoxP sites. These are specific sequences recognized by the Cre recombinase enzyme. - **GFP and RFP:** GFP is followed by the red fluorescent protein (RFP) gene. Here, it is important to note that both the GFP and RFP genes do not have their own start codons; they rely on the start codon provided at the beginning of the sequence. - **Expression:** Downstream of the RFP gene is an unspecified sequence that will be expressed only if Cre recombinase facilitates the removal of the sequence between the LoxP sites. **Cell Observation Conditions:** The activity of the constructs within cells is determined by visualizing them using a fluorescence microscope. Below are the expected results under specific conditions. 1. **Absence of Cre:** - **Image A:** This image displays no expression (black), indicating that without Cre recombinase, there is no activation of GFP or RFP genes. 2. **Cells treated with drug that induces expression of Cre:** - **Image B:** This image displays cells expressing only the GFP (green fluorescence). - **Image C:** This image shows cells expressing only RFP (red fluorescence). - **Image D:** This image reveals the simultaneous expression of both GFP and RFP (yellow/orange due to the combination of red and green fluorescences). **Matching Conditions with Expected Cell Observations:** - **Absence of Cre:** - Match with **Image A**, indicating no expression of GFP or RFP. - **Cells treated with Cre-inducing drug:** - Match with **Image B** for exclusive GFP expression. - Match with **Image C** for exclusive RFP expression. - Match with **Image D** for the
Transcribed Image Text:**Cre-Lox Reporter Gene System for Genetic Engineering** The Cre-Lox system is an advanced technique used for site-specific DNA modification in genetic engineering. This technology is particularly valuable for its precision in gene expression studies and the manipulation of genetic material. The following explanation provides an overview of a reporter gene construct set up to test the Cre-Lox system functionality within cells. **Construct Diagram Explanation:** - **CMV-Pro:** The construct starts with a Cytomegalovirus (CMV) promoter, a commonly used promoter in genetic engineering due to its high expression levels in mammalian cells. - **LoxP Sites:** Flanking the green fluorescent protein (GFP) gene are the LoxP sites. These are specific sequences recognized by the Cre recombinase enzyme. - **GFP and RFP:** GFP is followed by the red fluorescent protein (RFP) gene. Here, it is important to note that both the GFP and RFP genes do not have their own start codons; they rely on the start codon provided at the beginning of the sequence. - **Expression:** Downstream of the RFP gene is an unspecified sequence that will be expressed only if Cre recombinase facilitates the removal of the sequence between the LoxP sites. **Cell Observation Conditions:** The activity of the constructs within cells is determined by visualizing them using a fluorescence microscope. Below are the expected results under specific conditions. 1. **Absence of Cre:** - **Image A:** This image displays no expression (black), indicating that without Cre recombinase, there is no activation of GFP or RFP genes. 2. **Cells treated with drug that induces expression of Cre:** - **Image B:** This image displays cells expressing only the GFP (green fluorescence). - **Image C:** This image shows cells expressing only RFP (red fluorescence). - **Image D:** This image reveals the simultaneous expression of both GFP and RFP (yellow/orange due to the combination of red and green fluorescences). **Matching Conditions with Expected Cell Observations:** - **Absence of Cre:** - Match with **Image A**, indicating no expression of GFP or RFP. - **Cells treated with Cre-inducing drug:** - Match with **Image B** for exclusive GFP expression. - Match with **Image C** for exclusive RFP expression. - Match with **Image D** for the
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