Regulation of Genes and Their products 1. Given the following genotypes, explain how the mutation (identified by a (-) superscript) wil affect E. coll grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene products from the lac operon? What will be the implication of the missing gene product, if ever? Will the cell be able to survive in the lactose medium or not? a. I+p+o+z- y+ b. i- p+o+z+y+ c. i+p+o- z+y+ d. i+p- o+z+y+ 2. In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan. 3. Experiments show that mutations at gene E lead to non-repressible transcription of trp genes. Why?

Q: Consider a polycistronic operon consisting of genes B, C, and D (in that order) and with a single…

A: A polycistronic operon is a DNA segment that contains multiple genes that are transcribed into a…

Q: Molecular biology, please explain in detail

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Q: draw the p21 promoter. Your drawing should include (1) the start site, (2) the TATA box and (3) the…

A: p21 protein:  The p21 protein is a key protein that regulates the DNA repair process inside the…

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Q: Describe the lac operon

A: Lac operon, this concept was proposed by Jacob and Monod in 1965. An operon is a functioning unit of…

Q: 5g. A mutation has occurred rendering the regulatory protein unable to recognize the operator…

A: Operons are groups of genes involved in regulating a process and are transcribed as a single mRNA.…

Q: For each of the following items, describe what it is explain the necessity for each in cloning…

A: Genetic engineering is the application of technology in the manipulation of genetic information.…

Q: onsider a mutant of E. coli that has an inactivating mutation in the gene for the catabolite…

A: Catabolite activator protein (CAP) This protein senses the presence of glucose. It stimulates the…

Q: ble opposite shows the standard (coding strand) DNA codes for the 20 amino acids involved in protein…

A: Introduction :- Gene expression is the process of formation of response or functional part through a…

Q: 94 In the arrested state (7-14 nucleotides) of elongation,   ( ) is required to reactivate…

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A: Bacterial genetics is the science that helps to study the mechanism of hereditary in bacteria. The…

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A: Bacteria can transform in order to take up and integrate foreign DNA from their surroundings. A…

Q: UI ue bew strand requires the addition of a new dNTP to the 3 -OH g1 group of ovdes the immediate…

A: The synthesis of complementary DNA strand is called DNA replication.It is a very complex process…

Q: 3. Draw an anabolic operon with three biosynthetic genes for compound Q and a catabolic operon with…

A: ANSWER;-

Q: Similar to the Lac operon, the Ara (arabinose) operon genes are most highly expressed when Both…

A: Similar to the Lac operon, the Ara (arabinose) operon genes are most highly expressed when Cyclic…

Q: 4b. Briefly describe the changes in gene expression that happen in the lac operon when lactose is…

A: 4b- lac operon: - Inducible system. - Both positive and negative regulation. - Involved in lactose…

Q: Please ASAP. Thank you. What is the mechanism of action for CRE  recombinase in the CRE-loxP system

A: Cre recombinase is tyrosine based enzyme and is derived from P1 bacteriophage. It uses topoisomerase…

Q: Pls help ASAP. Use the info to ans the q.

A: Based on the information provided, the correct option would be::- the lac operator (lacO).The lac…

Q: 4. IPTG is an analog of lactose which is used to release the repressor on the lac operon allowing…

A: It can be defined as any alteration in a particular gene which gives rise to a different expression…

Q: 2. Assume you have identified a new operon in bacteria (which you call the suc operon) that encodes…

A: The gene expression in prokaryotes is under the control of operon system in which the transcription…

Q: Explain what would happen to the processes of protein synthesis if a molecule blocked the A-site of…

A: The protein synthesis is known as translation that involves production of polypeptide chain from the…

Q: The LEAST critical part of a plasmid needed to create a transgenic cow that expresses a foreign…

A: Transgenic cows are produced by DNA cloning methods in which a foreign gene is inserted into the…

Q: 4. A mutant strain of E. coli produces B-galactosidase in the presence and the absence of lactose.…

A: An operon is defined as a set of structural genes regulated by a common promoter in bacteria.

Q: Introducing the recombinant lac operon/mouse tyrosinase DNA into mice is only half of the equation…

A: Regulating tyrosinase is a significant aspect in various biological processes, especially those…

Q: What would be the best match? inducible operon, on O transcription is part of translation O…

A: Inducible operon is regulated by inducers which can on or off the operon for example lac operon is…

Q: 11. Using the information in the introduction on mutations and your knowledge of proteins, develop a…

A: The  gene provides instructions for making a protein called the melanocortin receptor that is :-…

Q: When a structural gene is under positive inducible control, what would be the result of a mutation…

A: An operon is a set of genes whose  functions are related to each other and are clustered together…

Q: You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?

A: Gene regulation is the process in which the DNA which contains information is transcribed into the…

Q: 1. What is the total size of the PMBBS plasmid in bp? Answer: bp 2. How many cut sites on the pMBBS…

A: As per the guidelines, we are supposed to answer only three subparts. Kindly repost the question…

Q: For your 25ul ligation reaction: You need to add 160ng of your digested insert DNA (which is at a…

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Q: regulation of the lac operon by cAMP-CAP complex is an example of positive regulation. what in…

A: They are transcription, translation, post-transcription and post-translation. Genes which code for…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: Introduction The lactose operon (also known as the lac operon) is a group of genes present in E.…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.