Describe the lac operon

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Topic Video
Question
**Title: Lac Operon Structure and Function**

**Introduction:**

The lac operon is a set of genes found in E. coli and other bacteria that is involved in the metabolism of lactose. This operon is a classic example of gene regulation in prokaryotic cells, demonstrating an inducible system where the presence of a substrate (lactose) activates gene expression.

**Components of the Lac Operon:**

1. **Promoter (P):** The site where RNA polymerase binds to initiate transcription of the operon.

2. **Operator (O):** A regulatory sequence where the repressor protein can bind to prevent transcription.

3. **Structural Genes:**
   - **lacZ:** Encodes β-galactosidase, an enzyme that breaks down lactose into glucose and galactose.
   - **lacY:** Encodes permease, a protein that facilitates the entry of lactose into the cell.
   - **lacA:** Encodes transacetylase, an enzyme with a less defined role in lactose metabolism but believed to be involved in detoxifying byproducts of lactose digestion.

4. **Regulatory Genes:**
   - **lacI:** Encodes the repressor protein that can bind to the operator to inhibit transcription.

**Mechanism of Action:**

- **In Absence of Lactose:** The repressor protein binds to the operator, blocking RNA polymerase from transcribing the lac genes.
   
- **In Presence of Lactose:** Lactose (or its isomer allolactose) binds to the repressor, causing a conformational change that prevents the repressor from binding to the operator. This allows RNA polymerase to transcribe the genes, leading to the production of enzymes that metabolize lactose.

**Diagram Description:**

A typical diagram of the lac operon includes:
- The DNA sequence with promoter, operator, and structural genes aligned linearly.
- Arrows indicating the transcriptional direction.
- Proteins like RNA polymerase and repressor depicted with their respective binding sites.
- Activation process detailing lactose effect on the repressor to demonstrate gene expression control.

**Conclusion:**

The lac operon is a vital model for understanding gene regulation mechanisms in prokaryotes, offering insights into more complex gene regulation systems in higher organisms.
Transcribed Image Text:**Title: Lac Operon Structure and Function** **Introduction:** The lac operon is a set of genes found in E. coli and other bacteria that is involved in the metabolism of lactose. This operon is a classic example of gene regulation in prokaryotic cells, demonstrating an inducible system where the presence of a substrate (lactose) activates gene expression. **Components of the Lac Operon:** 1. **Promoter (P):** The site where RNA polymerase binds to initiate transcription of the operon. 2. **Operator (O):** A regulatory sequence where the repressor protein can bind to prevent transcription. 3. **Structural Genes:** - **lacZ:** Encodes β-galactosidase, an enzyme that breaks down lactose into glucose and galactose. - **lacY:** Encodes permease, a protein that facilitates the entry of lactose into the cell. - **lacA:** Encodes transacetylase, an enzyme with a less defined role in lactose metabolism but believed to be involved in detoxifying byproducts of lactose digestion. 4. **Regulatory Genes:** - **lacI:** Encodes the repressor protein that can bind to the operator to inhibit transcription. **Mechanism of Action:** - **In Absence of Lactose:** The repressor protein binds to the operator, blocking RNA polymerase from transcribing the lac genes. - **In Presence of Lactose:** Lactose (or its isomer allolactose) binds to the repressor, causing a conformational change that prevents the repressor from binding to the operator. This allows RNA polymerase to transcribe the genes, leading to the production of enzymes that metabolize lactose. **Diagram Description:** A typical diagram of the lac operon includes: - The DNA sequence with promoter, operator, and structural genes aligned linearly. - Arrows indicating the transcriptional direction. - Proteins like RNA polymerase and repressor depicted with their respective binding sites. - Activation process detailing lactose effect on the repressor to demonstrate gene expression control. **Conclusion:** The lac operon is a vital model for understanding gene regulation mechanisms in prokaryotes, offering insights into more complex gene regulation systems in higher organisms.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Gene expression
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education