2. Assume you have identified a new operon in bacteria (which you call the suc operon) that encodes enzymes required for catabolism of the sugar sucrose. The structural genes that are required to breakdown sucrose are transcribed from a promoter (Psuc). One of these enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulator, called SucR, which binds to an operator (0). Using your knowledge of the lac operon, interpret the results of the following experiments (Table 1) to propose a model describing how the sucrose operon might be regulated. Cell genotype Production of SucS enzyme Without sucrose With sucrose R* O* p* s* R O* p* s* R* O P* s* R O* p* s* / R* Oʻ p* 5 ++ -- -- -- ++ -- R°O* P* S ++ ++ Table 1. Production of SucS enzyme in various strains. “-" in the cell genotype indicates a loss of function mutation, “+" indicates wild type function, two genotypes separated by a / indicate a merodiploid or partial diploid. R° is a mutant version of SucR that always binds to O. Production of SucS enzyme is indicated with “++", and no expression with “–". You may include diagrams in your answer.
2. Assume you have identified a new operon in bacteria (which you call the suc operon) that encodes enzymes required for catabolism of the sugar sucrose. The structural genes that are required to breakdown sucrose are transcribed from a promoter (Psuc). One of these enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulator, called SucR, which binds to an operator (0). Using your knowledge of the lac operon, interpret the results of the following experiments (Table 1) to propose a model describing how the sucrose operon might be regulated. Cell genotype Production of SucS enzyme Without sucrose With sucrose R* O* p* s* R O* p* s* R* O P* s* R O* p* s* / R* Oʻ p* 5 ++ -- -- -- ++ -- R°O* P* S ++ ++ Table 1. Production of SucS enzyme in various strains. “-" in the cell genotype indicates a loss of function mutation, “+" indicates wild type function, two genotypes separated by a / indicate a merodiploid or partial diploid. R° is a mutant version of SucR that always binds to O. Production of SucS enzyme is indicated with “++", and no expression with “–". You may include diagrams in your answer.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:2. Assume you have identified a new operon in bacteria (which you call the suc operon) that
encodes enzymes required for catabolism of the sugar sucrose. The structural genes that are
required to breakdown sucrose are transcribed from a promoter (Psuc). One of these
enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulator,
called SucR, which binds to an operator (O). Using your knowledge of the lac operon,
interpret the results of the following experiments (Table 1) to propose a model describing
how the sucrose operon might be regulated.
Cell genotype
Production of SucS enzyme
Without sucrose
With sucrose
R* O* P* s*
R O* p* s*
++
R* O P* S*
--
RO* p* s* / R* Oʻ p* 5
R° O* p* S
++
--
++
++
Table 1. Production of SucS enzyme in various strains. “-" in the cell genotype indicates a loss
of function mutation, “+" indicates wild type function, two genotypes separated by a /
indicate a merodiploid or partial diploid. R° is a mutant version of SucR that always binds to
O. Production of SucS enzyme is indicated with “++", and no expression with “–". You may
include diagrams in your answer.
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