LACTOSE .how will you inoculate? .How will you incubate? . what are Post- Incubation procedures? . How will you read the positive and negative results?
LACTOSE
.how will you inoculate?
.How will you incubate?
. what are Post- Incubation procedures?
. How will you read the positive and negative results?
Fermentative degradation of lactose(Disaccharide) by microbe, under anaerobic condition is carried out in a fermentation tube.
A fermentation tube is a culture tube that contains a Durham tube (a small tube placed in an inverted position in the culture tube) for the detection of gas production, as an end product of metabolism. The fermentation broth contains ingredients of nutrient broth, a specific carbohydrate(glucose, lactose), and a pH indicator (phenol red), which is red at a neutral pH(7) and turns yellow at or below a pH of 6.8 due to the production of an organic acid.
Step by step
Solved in 3 steps
