Given 1mL of Bovine Serum Albumin (BSA) at known concentration 2mg/mL for the standard curve. Calculate 5 standrard BSA dilution with water in the range of 20 - 2000 μg/mL.
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- KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)Fill in the volumes required to make the standard glucose solutions of various conentrations provided. provide calculations as well.
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- Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.The order is for Streptomycin 1gm IM. You have a 5gm vial of Streptomycin . The label states to add 9ml of sterile water to yield 400mq / m * l How many ml will you give?The order reads: Give mannitol 0.5 g/kg IV now, over 2 hours. The patient weighs 165 lb, and you have a 100-mL vial of 20% mannitol. How many grams will the patient receive? How many milliliters of mannitol will you prepare for this infusion?
- The doctor ordered Claforan 1400mg IM every 12 hours. The directions for the 2 gm vial state: Add 5 mL of diluent to yield an approximate concentration of 360mg/mL. How many mL will you give?In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?