Based on the Agarose Gel Electrophoresis results, tell in which concentration range of KCl the restriction enzyme ECORI eluted in the DEAE Cellulose Anion Exchange Chromatography Step.
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- Besides agarosc gel electrophoresis, there is also SDS-PAGE for separation of macromolccules. Differentiate both these methods.Iryour igation and transformation reactions were successful, what will you see on the LB/AMP/X-gal/IPTC agar plate? OWhite growth O Blue growth O No growthFollowing electrophoresis of the two pGlo mini-preparation samples (both digested and undigested), the following agarose gel image was obtained. provide an accurate and detailed figure, describing the image and what it depicts.
- a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.Briefly describe the principle behind agarose gel electrophoresis with reference to the pore sizes. Indicate the percentage gel used to separate the digested fragments and provide a reason as to why this % was selected.Why is GAPDH not be a suitable loading control for nuclear extracts?
- Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of CentrifugationAfter adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?