Based on the Agarose Gel Electrophoresis results, tell in which concentration range of KCl the restriction enzyme ECORI eluted in the DEAE Cellulose Anion Exchange Chromatography Step.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Experimental Data:
1 ml
1 ml
1 ml
1 ml
1 ml
1 ml
1 ml
1 ml
1 ml
Tube 1
Distilled water
Tube 2
Buffer
Tube 3
Buffer
Tube 4
Buffer
Tube 5
KCI
0.1
Tube 6
KCI
0.1
Tube 7
KCI
0.2
Tube 8
KCI
0.2
Tube 9
KCI
0.5
Agarose Gel:
Lane 1
Marker
Lane 2
Tube 2
Lane 3
Tube 3
Lane 4
Tube 4
Lane 5
Tube 5
Lane 6
Tube 6
Lane 7
Tube 7
Lane 8
Tube 8
Tube 1 and 9, do not apply.
Transcribed Image Text:Experimental Data: 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml Tube 1 Distilled water Tube 2 Buffer Tube 3 Buffer Tube 4 Buffer Tube 5 KCI 0.1 Tube 6 KCI 0.1 Tube 7 KCI 0.2 Tube 8 KCI 0.2 Tube 9 KCI 0.5 Agarose Gel: Lane 1 Marker Lane 2 Tube 2 Lane 3 Tube 3 Lane 4 Tube 4 Lane 5 Tube 5 Lane 6 Tube 6 Lane 7 Tube 7 Lane 8 Tube 8 Tube 1 and 9, do not apply.
Based on the Agarose Gel Electrophoresis
results, tell in which concentration range of
KCl the restriction enzyme EcoRI eluted in the
DEAE Cellulose Anion Exchange
Chromatography Step.
Transcribed Image Text:Based on the Agarose Gel Electrophoresis results, tell in which concentration range of KCl the restriction enzyme EcoRI eluted in the DEAE Cellulose Anion Exchange Chromatography Step.
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