Your tyrosinase enzyme assay results in a slope of 0.001946 sec-¹ for absorbance at 475 nm. Give the relative activity of your undiluted crude extract if 30.00 µL of a 5x dilution of the crude extract was added to a 1.00 mL reaction cuvette in performing the assay. Hint: Look at the data analysis section of the lesson for help in solving this problem. 5.41 U/mL 7.72 U/mL 1.08 U/mL 0.216 U/mL
Q: One of the reasons for oxidizing the disulfide bonds in RNase A before removing the urea in the…
A: In the Anfinsen experiment, Christian Anfinsen aimed to investigate the relationship between the…
Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…
A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…
Q: Question 5 Listen Paul, has hemophilia, a sex linked recessive condition. Which of the following is…
A: The objective of the question is to determine the correct genotype for Paul who has hemophilia, a…
Q: Please answer the following answer: Ethanol metabolism has been part of the human diet for…
A: The question is asking about the effects of high concentration of NADH, a product of ethanol…
Q: Which pathways are stimulated by glucagon? Select all that apply. glycogen synthesis glycogen…
A: The objective of the question is to identify the metabolic pathways that are stimulated by the…
Q: Amino acid structure For any ionizable group, indicate the pka value and draw the structure that…
A: All ionizable groups have their characteristic pKa value. pKa is the pH at which half of all the…
Q: A disaccharide that is found in dairy products can be formed by the two monosaccharides. Please draw…
A: The organic molecules known as carbohydrates are made up of three elements: carbon, hydrogen, and…
Q: b. Write a paragraph or bullet point list to go along with the figure 11.8b describing the role of…
A: Standard change in Gibbs free energy ( ) of redox reactions can be found, if we know the standard…
Q: The conjugate base of diethyl malonate can serve as a nucleophile to attack a wide range of…
A: In diethyl malonate the CH2 carbon hydrogens are more acidic they are abstracted by base to form a…
Q: Which of the following statements is not true about the catalytic triad in chymotrypsin? Group of…
A: The objective of the question is to identify the incorrect statement about the catalytic triad in…
Q: pH 14 12 10 H₂N-CH 8 6 4 2 доон PK1 çoo- H3N-CH CH₂ CH₂ CH₂ CH2 NH3* pK₁ 0 0.5 1 CH₂ CH₂ CH2 CH₂…
A: pH = -log10[H+], where [H+] is the concentration of hydrogen ions in the solution.Logarithm measures…
Q: Indicate the hinge region, GK kinaseselectivity pocket and explain "Solvent AccessibilitySurface"…
A: The hinge region in a protein kinase is a critical part of the enzyme that connects the N-terminal…
Q: r^2=6Dt , where D is the diffusion coefficient of thediffusing object and t is the time that the…
A: The objective of the question is to find out the time it takes for a protein to diffuse across a…
Q: You perform a size exclusion chromatography experiment and generate the following standard curve.…
A: In the question Kav vs molecular weight plot is given and the resulting linear equation is: y =…
Q: Throughout the semester we have seen that regulation is a key aspect of Biochemistry. It affects all…
A: Biochemical reactions and metabolic pathways are very important to be regulated as it enables the…
Q: Calculate AG for the reaction G + H I + J when [G] = 0.0132 mM, [H] = 35.1 uM, [1] = 55.6 uM, [J]…
A: Since conditions inside the cell are different than standard temperature and pressure, biochemists…
Q: In a Lineweaver-Burk graph, the place where the line crosses the Y-axis represents: a) Km b) Vmax…
A: The LB graph is a secondary plot of the Michaelis-Menten (MM) graph. The LB graph has 1/[S0] as…
Q: Mutations leading to changes that can be inherited by the next generation have be introduced at the…
A: There are four classes of biological macromolecules: nucleic acid, proteins, lipids and…
Q: fatty acid
A: Given Structure:The provided structure represents a fatty acid featuring an omega-3 double…
Q: Using the graph paper provided, plot the velocity of the reaction (v) against the concentration of…
A: Michaelis Menten (MM) plot has [S] as X-axis and V as Y-axis. Here we are given the 1/[S] and 1/V…
Q: How does the coplanar nature of the peptide bond impact the overall structure of D O a. a. It…
A: A peptide bond is an amide type of covalent bond linking two consecutive alpha-amino acids as shown…
Q: 2) Fill in the blank by selecting one of the options below: a. Primary structure. b. Secondary…
A: The correct answer is: b. Secondary structure."the structure formed by hydrogen bonds between…
Q: Draw the complete chemical structure of one ATP molecule at physiological pH. (b) In your drawing,…
A: ATP is the energy currency that a cell spends on metabolic transactions. It is a nucleotide made up…
Q: Which of the following compounds is most similar to the transition state of peptide bond hydrolysis…
A: "peptide hydrolysis" is defined as the breaking of peptide bind by adding water molecule to the…
Q: Matching :1. MDR protein Part of NAD molecule ______ 2. SGLT Part of FAD molecule _____ 3. Grb-2…
A: The objective of the question is to correctly match the biochemical entities with their respective…
Q: Which analogue involves a retroinverso peptide isostere? CH₂ A с CH3 Ö G O NH FX NH S OH OH S B D S…
A: Retro-inverse peptide:1. The sequence of parent chain is reversed such that the N side to C side…
Q: True or False: The presence of Howell-Jolly bodies on a blood smear is indicative of asplenia or…
A: Howell-Jolly bodies are clusters of DNA which are nothing but basophilic nuclear remenents present…
Q: Question 2
A: The objective of this question is to determine the genotypes of the parent pea plants based on the…
Q: Predict the most likely product of this biochemical reaction: HO NH3 + Pi X + H₂O
A: Here we are given a phosphorylation reaction. The phosphate donor here is inorganic phosphate (Pi).…
Q: odd-chain fatty acids are metabolized down to propionyl-CoA (a 3 Carbon unit). This is converted in…
A: Fatty acids are fundamental units that make up fats, also known as lipids. During the process of…
Q: Which of the following regarding DNA replication is FALSE. Replication of DNA is highly regulated.…
A: There are four classes of biological macromolecules: Nucleic acids, proteins, lipids and…
Q: Which of the following statements are true? (This is a multi- select question, select all that…
A: There are four classes of biological macromolecules: nucleic acid, proteins, lipids and…
Q: Differentiate the advantages and disadvantages of messenger ribonucleic acid (mRNA) by RT-PCR and…
A: Cytokines are small signaling proteins that undertake both paracrine and endocrine signaling by…
Q: Draw the chemical structure of an alanine pentapeptide. Indicate the location of each peptide bond.…
A: Amino acids are biomolecules that have a carboxyl group, an amino group, a hydrogen atom and a…
Q: Carbohydrates: Draw the structures of the two products obtained when sucrose is subjected to…
A: Sucrose is a disaccharide composed of a and an . The C1 of forms a glycosidic bond with C2 of in…
Q: Glucose 1-phosphate formed by glycogen degradation is converted to glucose 6-phosphate by…
A: Glycogen is a stored form of glucose and is reserve food material in animals. Glycogenolysis is a…
Q: (a) The mean residence time of any single component i is given by "₁ L UE (1-8)(1+K₁) where u is…
A: (a) Mean Residence Time :The mean residence time is the average time a solute molecule spends in…
Q: H OH OH HO OH HO- two disaccharides H two monosaccharides and one disaccharide four monosaccharides…
A: Beta galactosidase is an enzyme which cleaves the beta glycosidic bond between galactose and…
Q: This is the question: Ethanol metabolism has been part of the human diet for centuries. Ethanol…
A: The question is asking about the effects of high concentration of NADH, a product of ethanol…
Q: 2 SCOA 1) fatty acid synthase (1 cycle) 2) NADH reductase all in water
A: The image shows a chemical reaction. The reactants are fatty acid synthase (1cycle) and NADH…
Q: Which of the twenty naturally occurring amino acid side chains are charged at a pan of 1.00? pH of…
A: Amino acids can be defined as organic compounds which comprise of both amino & carboxylic acid…
Q: I. True or False. Write the word “reducing” if the statement is correct, otherwise write…
A: The objective of the question is to determine whether each statement is true or false in the context…
Q: The biosynthesis of vitamin D₂ is a two-step reaction, requiring two types of concerted pericyclic…
A: Biosynthesis of Vitamin D2 (ergocalciferol) is a two-step reaction. Step (i) is a photochemical ring…
Q: If aspartate is labeled with deuterium D (= H-2 = 12H), answer questions a-c to determine where the…
A: The objective of the question is to determine the position of the deuterium label in oxaloacetate…
Q: Which coenzyme is most likely used in the following biochemical reaction? çoo L CH COO C CH₂ I CH₂ L…
A: Tricarboxylic Acid Cycle:It is also known as the TCA cycle or Citric Acid Cycle or the Krebs…
Q: The following are sequences from three different alpha helices found in human proteins:…
A: The proteins are constituted of twenty naturally occurring amino acids connected via peptide…
Q: What is the effect of the following mutations of succinate Dehydrogenase/Complex II? Would you…
A: The objective of the question is to understand the effect of specific mutations in the succinate…
Q: Match each of the following aspects of DNA with the main structural feature which makes it possible.…
A: DNA (including the B-DNA) has a major and a minor groove. The major groove is wider compared to the…
Q: 1. Amino acid side chains act as nucleophiles and proton donor/acceptors in various enzymatic…
A: Peptides are composed of amino acids. Some amino acids have ionizable side chains while others do…
Q: A high concentration of which of the following would activate the citric acid cycle? ADP ATP Acetyl…
A: In the animal tissue, the citric acid cycle takes place in the mitochondrial matrix. Acetyl CoA from…
Please dont provide handwrittin solution.../.
Step by step
Solved in 3 steps with 17 images
- You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).This graph represents a standard curve from a BCA assay for protein concentration. If your BCA reaction with your unknown protein has an absorbance of 0.2, approximately what concentration of protein is in your unknown sample? Absorbance (562nm) 0.5 0.45 3 0.35 0.3. 0.25 0.15. 0.1- 0.05 ~0.1 μg/ml ~4.5 μg/ul less that 0.005 | 0.2 μg/μ. [Protein (BSA)) (pg/ml)
- A 0.5 M phosphate buffer at pH 6.5 and a 10 mM substrate stock solution are available for preparing enzyme assays. Each assay must have a total volume of 3 mL and a final phosphate concentration of 100 mM is desired. Using the substrate concentration of 3.33 mM and 100 µL of the enzyme solution, calculate the volume of buffer, volume of substrate stock solution, and volume of water that should be place in a cuvette for the assay.You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction mixture and measure a slope of 0.125 abs/min in an LDH assay. What is the reaction rate in uM/min? Show your work.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.Concentrations in biochemical systems are often very dilute. Consequently, scientific notation and logarithms are often used to express concentrations. In scientific notation, numbers are expressed as coefficient x 10" To convert a number to scientific notation, proceed as follows: 1. Move the decimal place so that there is one digit in front of the decimal. 2. Account for the moved decimal in the value of x. If the decimal moved to the right, x is negative; if it moved to the left, x is positive. A logarithm is basically an exponent. Unless otherwise indicated, a logarithm is the a of 10". The numbers after the decimal point are significant; the number before the decimal just identify the location of the decimal point for the number. Notice that it is easy to estimate a logarithm from scientific notation; it's the exponent! Logarithms are commonly used to express the concentration of H. The pH is defined as pH= log (In), where the base number is 2.303. The same general rules as logs…Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XA series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL solution of bovine gamma globulin (BGG). From these solutions, you will need to make 100 μL of each of the following standards using 1x MBP purification buffer as the diluent for preparation of a standard curve: 1000 μg/mL, 750 μg/mL, 500 μg/mL, 250 μg/mL, 125 μg/mL, and 0 μg/mL.