How many amino acids make up the unknown protein? How many nucleotides in the mRNA transcript? How many nucleotides in the gene?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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How can I do this table in red please
Unknown A
MW (KD)
Unknown B
MW (KD)
Body*
#C
How many amino acids make up the unknown protein?
How many nucleotides in the mRNA transcript?
How many nucleotides in the gene?
$
Amino acids
Amino acids
A
B
%
I
nucleotides
6
nucleotides
MacBook Pro
7
Transcribed Image Text:Unknown A MW (KD) Unknown B MW (KD) Body* #C How many amino acids make up the unknown protein? How many nucleotides in the mRNA transcript? How many nucleotides in the gene? $ Amino acids Amino acids A B % I nucleotides 6 nucleotides MacBook Pro 7
yer of plastic wrap.
3. Measure the distance travelled by the dye front. DYE FRONT =
mm
4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in
your ladder. Measure from the bottom of the well to each band.
5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns.
Protein Ladder (for trendline graph):
Migration
Distance
(mm)
Rf=
Band Migration
Dye Front Migration
0-074
17
22.
28.
-22335
34
Measurements for Unknown
Migration Distance (mm)
4
0-111
0-166
0.203
0.314
0-407
Disig
0.574
0.759,
ما
14
Measurements for Unknown
Migration Distance (mm)
Molecular Weight
(in KD)
A
A THURST
Rf
6074
260 250.
Rf
315
50
這
15
10
BEHJUL
kDa
--260-
--140-
--100-
-70
-50
-40
-35
-25
-15
-10
Log of
Molecular Weight
2.415
Estimated Molecular Weight
Estimated Molecular Weight
Transcribed Image Text:yer of plastic wrap. 3. Measure the distance travelled by the dye front. DYE FRONT = mm 4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in your ladder. Measure from the bottom of the well to each band. 5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns. Protein Ladder (for trendline graph): Migration Distance (mm) Rf= Band Migration Dye Front Migration 0-074 17 22. 28. -22335 34 Measurements for Unknown Migration Distance (mm) 4 0-111 0-166 0.203 0.314 0-407 Disig 0.574 0.759, ما 14 Measurements for Unknown Migration Distance (mm) Molecular Weight (in KD) A A THURST Rf 6074 260 250. Rf 315 50 這 15 10 BEHJUL kDa --260- --140- --100- -70 -50 -40 -35 -25 -15 -10 Log of Molecular Weight 2.415 Estimated Molecular Weight Estimated Molecular Weight
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