After finishing running IEX on HPLC, you found out that the detector on HPLC was not on during purification. Fraction collector collected all the fractions as expected. Describe assay or assays you would have to do for generating a chromatogram for your lab report?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
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Chapter2: Basic Hematology
Section2.13: Erythrocyte Sedimentation Rate
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After finishing running IEX on HPLC, you
found out that the detector on HPLC was
not on during purification. Fraction
collector collected all the fractions as
expected.
Describe assay or assays you would have
to do for generating a chromatogram for
your lab report?
Transcribed Image Text:After finishing running IEX on HPLC, you found out that the detector on HPLC was not on during purification. Fraction collector collected all the fractions as expected. Describe assay or assays you would have to do for generating a chromatogram for your lab report?
If there are still contaminating proteins
with your LDH after going through
affinity and lon exchange
chromatography.
what would their physical characteristics
be?
Transcribed Image Text:If there are still contaminating proteins with your LDH after going through affinity and lon exchange chromatography. what would their physical characteristics be?
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