After finishing running IEX on HPLC, you found out that the detector on HPLC was not on during purification. Fraction collector collected all the fractions as expected. Describe assay or assays you would have to do for generating a chromatogram for your lab report?
After finishing running IEX on HPLC, you found out that the detector on HPLC was not on during purification. Fraction collector collected all the fractions as expected. Describe assay or assays you would have to do for generating a chromatogram for your lab report?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![After finishing running IEX on HPLC, you
found out that the detector on HPLC was
not on during purification. Fraction
collector collected all the fractions as
expected.
Describe assay or assays you would have
to do for generating a chromatogram for
your lab report?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd9036ccd-b518-40e3-89ff-336d4c576363%2F4d818a29-ab0d-4ebb-bbeb-c2cbd5a40d54%2Fztcozq8_processed.jpeg&w=3840&q=75)
Transcribed Image Text:After finishing running IEX on HPLC, you
found out that the detector on HPLC was
not on during purification. Fraction
collector collected all the fractions as
expected.
Describe assay or assays you would have
to do for generating a chromatogram for
your lab report?
![If there are still contaminating proteins
with your LDH after going through
affinity and lon exchange
chromatography.
what would their physical characteristics
be?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd9036ccd-b518-40e3-89ff-336d4c576363%2F4d818a29-ab0d-4ebb-bbeb-c2cbd5a40d54%2Ffgu57f7_processed.jpeg&w=3840&q=75)
Transcribed Image Text:If there are still contaminating proteins
with your LDH after going through
affinity and lon exchange
chromatography.
what would their physical characteristics
be?
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