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Dilution Problem. A culture was diluted as follows: (1) 50mL are added to 100mL of water. (2) 1mL from (1) are then added to 9mL of water. (3) 100µL from (2) are then added to 9.9mL of water. (4) 100µL from (3) are plated for a pour plate and incubated. There were 135 colonies counted on one half of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many colonies would have been present if 1mL of the dilution was used for the pour plate? d) How much water would be needed for a 10-² dilution if the culture added was 1000uL?

Dilution Problem. A culture was diluted as follows: (1) 50mL are added to 100mL of water. (2) 1mL from (1) are then added to 9mL of water. (3) 100µL from (2) are then added to 9.9mL of water. (4) 100µL from (3) are plated for a pour plate and incubated. There were 135 colonies counted on one half of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many colonies would have been present if 1mL of the dilution was used for the pour plate? d) How much water would be needed for a 10-² dilution if the culture added was 1000uL?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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how do i complete part c and d? 

Dilution Problem. A culture was diluted as follows: (1) 50mL are added to 100mL of water. (2) 1mL from (1) are then added to 9mL of water. (3) 100µL from (2) are then added to 9.9mL of water. (4) 100uL from (3) are plated for a pour plate and incubated. There were 135 colonies counted on one half of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many colonies would have been present if 1mL of the dilution was used for the pour plate? d) How much water would be needed for a 10-2 dilution if the culture added was 1000uL?
Transcribed Image Text:Dilution Problem. A culture was diluted as follows: (1) 50mL are added to 100mL of water. (2) 1mL from (1) are then added to 9mL of water. (3) 100µL from (2) are then added to 9.9mL of water. (4) 100uL from (3) are plated for a pour plate and incubated. There were 135 colonies counted on one half of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? c) How many colonies would have been present if 1mL of the dilution was used for the pour plate? d) How much water would be needed for a 10-2 dilution if the culture added was 1000uL?
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