The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence codes for a single protein. Design 5’ and 3’ PCR primers that will amplify ONLY the open reading frame of the genes. Give these sequences in a 5’ to 3’ orientation. Include EcoR1 and NotI restriction enzyme recognition sites on the 5’ ends of the 5’ and 3’ primers respectively.
The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence codes for a single protein. Design 5’ and 3’ PCR primers that will amplify ONLY the open reading frame of the genes. Give these sequences in a 5’ to 3’ orientation. Include EcoR1 and NotI restriction enzyme recognition sites on the 5’ ends of the 5’ and 3’ primers respectively.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this
sequence codes for a single protein. Design 5’ and 3’ PCR primers that will amplify ONLY the open
reading frame of the genes. Give these sequences in a 5’ to 3’ orientation. Include EcoR1 and NotI
restriction enzyme recognition sites on the 5’ ends of the 5’ and 3’ primers respectively.
Transcribed Image Text:>Gene Z
AATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGAGTAGTAGGTCTTTGGCATTAGGAGCTTGAGCCCAGACGGCCCTAGCAGGGACCCCAGCGCCCGAGAGACCATGCAGAGGTCGC
CTCTGGAAAAGGCCAGCGTTGTCTCCAAACTTTTTTTCAGCTGGACCAGACCAATTTTGAGGAAAGGATACAGACAGCGCCTGGAATTGTCAGACATATACCAAATCCCTTCTGTTGATTCTGCTGACAATCTATCTGAAAAATT
GGAAAGAGAATGGGATAGAGAGCTGGCTTCAAAGAAAAATCCTAAACTCATTAATGCCCTTCGGCGATGTTTTTTCTGGAGATTTATGTTCTATGGAATCTTTTTATATTTAGGGGAAGTCACCAAAGCAGTACAGCCTCTCTTA
CTGGGAAGAATCATAGCTTCCTATGACCCGGATAACAAGGAGGAACGCTCTATCGCGATTTATCTAGGCATAGGCTTATGCCTTCTCTTTATTGTGAGGACACTGCTCCTACACCCAGCCATTTTTGGCCTTCATCACATTGGAA
TGCAGATGAGAATAGCTATGTTTAGTTTGATTTATAAGAAGACTTTAAAGCTGTCAAGCCGTGTTCTAGATAAAATAAGTATTGGACAACTTGTTAGTCTCCTTTCCAACAACCTGAACAAATTTGATGAAGGACTTGCATTGGC
ACATTTCGTGTGGATCGCTCCTTTGCAAGTGGCACTCCTCATGGGGCTAATCTGGGAGTTGTTACAGGCGTCTGCCTTCTGTGGACTTGGTTTCCTGATAGTCCTTGCCCTTTTTCAGGCTGGGCTAGGGAGAATGATGATGAAG
TACAGAGATCAGAGAGCTGGGAAGATCAGTGAAAGACTTGTGATTACCTCAGAAATGATTGAAAATATCCAATCTGTTAAGGCATACTGCTGGGAAGAAGCAATGGAAAAAATGATTGAAAACTTAAGACAAACAGAACTGAAAC
TGACTCGGAAGGCAGCCTАTGTGAGATАСТТСААТАGCTCAGCCTTСТТСТТСТСAGGGTTCTTTGTGGTGTTTТТАТСТGTGСTTСССТАТGCACTAATCААAGGAATCAТССТССGGAАААТАТТСАССАССАТСТСАТТСTG
САTTGTTCTGCGCATGGCGGTCACTCGGCAATTTCCOTGGGCTGTAСАААСАТGGТАTGACTCTСТTGGAGCAAТАAАСАAAАТАСAGGATTТСТТАСААААGCAAGAATAТАAGACATTGGAATATAAСТТААСGACTАСAGAA
GTAGTGATGGAGAATGTAАCAGCCTTCTGGGAGGAGGGATTTGGGGAATTATTTGAGAAAGCAAААСАAAАСААТААСААТAGAAAAACTTCТААTGGTGATGACAGCCTCТТСТТCAGTААТТТСТСАСТТСТTGGTAСТССТG
TCCTGAAAGATATTAATTTCAAGATAGAAAGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGAGCAGGCAAGACTTCACTTCTAATGGTGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCACAGTGGAAGAAT
TТСАТТСТGTTCTCAGTTТТССTGGATТАTGCCTGGCАССАТТАААGAAAATATCАТСТТTGGTGTTTCCTАTGATGAATATAGATACAGAAGCGTCATCAAAGCATGCCAАСТAGAAGAGGACATCTCСАAGTTTGCAGAGAAA
GACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCAAGAGCAGTATACAAAGATGCTGATTTGTATTTATTAGACTCTCCTTTTGGATACCTAGATGTTTTAACAGAAAAAG
AAATATTTGAAAGCTGTGTCTGTAAACTGATGGCTAACAAAACTAGGATTTTGGTCACTTCTAAAATGGAACATTTAAAGAAAGCTGACAAAATATTAATTTTGCATGAAGGTAGCAGCTATTTTTATGGGACATTTTCAGAACT
ССААААТСТАCAGCCAGAСТТТAGCTCААAАСТСАТGGGATGTGATТСТТТCGACСААТТТAGTGCAGAAAGAAGAAATTCAAТССТААСТGAGACCTTACAСCGTTTCTCАТTAGAAGGAGATGCTCCTGTCTCCTGGACAGAA
АСАААААААСААТСТТТТАААСAGACTGGAGAGTTTGGGGAAAAAAGGAAGAATTСТАТТСТСААТССААТСААСТСТАТАСGAAAATTTTCСАТTGTGCAAАAGACTCCCТТАСАААТGААTGGCATCGAAGAGGATTCTGATG
AGCCTTTAGAGAGAAGGCTGTCCTTAGTACCAGATTCTGAGCAGGGAGAGGCGATACTGCCTCGCATCAGCGTGATCAGCACTGGCCCCACGCTTCAGGCACGAAGGAGGCAGTCTGTCCTGAACCTGATGACACACTCAGTTAA
ССАAGGTCAGAACATTCAСCGAAAGACААСAGCATCСАСАCGAAAAGTGTCACTGGCCCСТCAGGCAAACTTGAСTGAACTGGATATATATTСАAGAAGGTTАТСТСААGAААСTGGCTTGGAAATAAGTтGAAGAAATTААCGAA
GAAGACTTAAAGGAGTGCTTTTTTGATGATATGGAGAGCATACCAGCAGTGACTACATGGAACACATACCTTCGATATATTACTGTCCACAAGAGCTTAATTTTTGTGCTAATTTGGTGCTTAGTAATTTTTCTGGCAGAGGTGG
СTGCTTСТТTGGTTGTGCTGTGGCTCCTTGGAAACACТССТСТТСАAGACAAAGGGAATAGTACTСАТAGTAGAААТААСАGCТАTGCAGTGАТТАТСАССАGCACCAGTTCGTATTATGTGTTTTACATТТАCGTGGGAGTAGC
CGACACTTTGCTTGCTATGGGATTCTTCAGAGGTCTАССАСТGGTGCATACTCТААТСАСАGTGTCGAAAATTTТАСАССАСААААTGTTACАТТСTGTTCTТСАAGCACCТАTGTCAACССТСААСАСGTTGAAAGCAGGTGGG
АТТСТТААТAGATTCTCCААAGATATAGCAATTTTGGATGACCTTCTGCCTСТТАССАТАТТТGАСТТСАТССАGTTGTTATTААТTGTGATTGGAGCTATAGCAGTTGTCGCAGTTTTAСААСССТАСАТСТТTGTTGCAACAG
TGCCAGTGAТAGTGGCTTТТАТТАТGTTGAGAGCATAТТТССТССАААССТСАСАGCAACTCAAАСААСТGGAAТСTGAAGGCAGGAGTCCAАТТТТСАСТСАТСТTGTTAСАAGCTTAAАAGGACTATGGACACTTCGTGCCTT
CGGACGGCAGCCTTACTTTGAAACTCTGTTCCACAAAGCTCTGAATТТАСАТАСТGCCAACTGGTТСTTGTACCTGTCAACACTGCGCTGGTТССАААТGAGAATAGAAATGATTTTTGTСАТСТТСТТСАТTGCTGTTАССТТС
ATTTCCATTTTAACAACAGGAGAAGGAGAAGGAAGAGTTGGTATTATCCTGACTTTAGCCATGAATATCATGAGTACATTGCAGTGGGCTGTAAACTCCAGCATAGATGTGGATAGCTTGATGCGATCTGTGAGCCGAGTCTTTA
AGTTCATTGACATGCCAACAGAAGGTAААССТАССААGTCAACCAAАССАТАСААGAATGGCCAАСТСТСGAAAGTTATGATTАТTGAGAATТСАСАCGTGAAGAAAGATGACATCTGGCССТСAGGGGGCCAAATGACTGTCAA
AGATCTCACAGCAAAATAСАСAGAAGGTGGAAATGCOАТАТТAGAGAACATTTCCТТСТСААТААGTCCTGGCCAGAGGGTGGGCCTСTTGGGAAGAACTGGATCAGGGAAGAGTACTTTGTTATCAGCTTTTTTGAGAСТАСТG
AACACTGAAGGAGAAATCCAGATCGATGGTGTGTCTTGGGATTCAATAACTTTGCAACAGTGGAGGAAAGCCTTTGGAGTGATACCACAGAAAGTATTTATTTTTTCTGGAACATTTAGAAAAAACTTGGATCCCTATGAACAGT
GGAGTGATCAAGAAATATGGAAAGTTGCAGATGAGGTTGGGCTCAGATCTGTGATAGAACAGTTTCCTGGGAAGCTTGACTTTGTCCTTGTGGATGGGGGCTGTGTCCTAAGCCATGGCCACAAGCAGTTGATGTGCTTGGCTAG
ATCTGTTCTCAGTAAGGCGAAGATCTTGCTGCTTGATGAACCCAGTGCTCATTTGGATCCAGTAACATACCAAATAATTAGAAGAACTCTAAAACAAGCATTTGCTGATTGCACAGTAATTCTCTGTGAACACAGGATAGAAGCA
ATGCTGGAATGCCAACAATTTTTGGTCATAGAAGAGAACAAAGTGCGGCAGTACGATTCCATCCAGAAACTGCTGAACGAGAGGAGCCTCTTCCGGCAAGCCATCAGCCCCTCCGACAGGGTGAAGCTCTTTCCCCACCGGAACT
CAAGCAAGTGCAAGTCTAAGCCCCAGATTGCTGCTCTGAAAGAGGAGACAGAAGAAGAGGTGCAAGATACAAGGCTTTAGAGAGCAGCATAAATGTTGACATGGGACATTTGCTCATGGAATTGGAGCTCGTGGGACAGTCACCT
CATGGAATTGGAGCTCGTGGAACAGTTACCTCTGCCTCAGAAAACAAGGATGAATTAAGTTTTTTTTTAA
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