The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence codes for a single protein. Design 5’ and 3’ PCR primers that will amplify ONLY the open reading frame of the genes. Give these sequences in a 5’ to 3’ orientation. Include EcoR1 and NotI restriction enzyme recognition sites on the 5’ ends of the 5’ and 3’ primers respectively.
The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence codes for a single protein. Design 5’ and 3’ PCR primers that will amplify ONLY the open reading frame of the genes. Give these sequences in a 5’ to 3’ orientation. Include EcoR1 and NotI restriction enzyme recognition sites on the 5’ ends of the 5’ and 3’ primers respectively.
Chapter4: Management Practices For Finfish
Section: Chapter Questions
Problem 23SA
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The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this
sequence codes for a single protein. Design 5’ and 3’ PCR primers that will amplify ONLY the open
reading frame of the genes. Give these sequences in a 5’ to 3’ orientation. Include EcoR1 and NotI
restriction enzyme recognition sites on the 5’ ends of the 5’ and 3’ primers respectively.
Transcribed Image Text:>Gene Z
AATTGGAAGCAAATGACATCACAGCAGGTCAGAGAAAAAGGGTTGAGCGGCAGGCACCCAGAGTAGTAGGTCTTTGGCATTAGGAGCTTGAGCCCAGACGGCCCTAGCAGGGACCCCAGCGCCCGAGAGACCATGCAGAGGTCGC
CTCTGGAAAAGGCCAGCGTTGTCTCCAAACTTTTTTTCAGCTGGACCAGACCAATTTTGAGGAAAGGATACAGACAGCGCCTGGAATTGTCAGACATATACCAAATCCCTTCTGTTGATTCTGCTGACAATCTATCTGAAAAATT
GGAAAGAGAATGGGATAGAGAGCTGGCTTCAAAGAAAAATCCTAAACTCATTAATGCCCTTCGGCGATGTTTTTTCTGGAGATTTATGTTCTATGGAATCTTTTTATATTTAGGGGAAGTCACCAAAGCAGTACAGCCTCTCTTA
CTGGGAAGAATCATAGCTTCCTATGACCCGGATAACAAGGAGGAACGCTCTATCGCGATTTATCTAGGCATAGGCTTATGCCTTCTCTTTATTGTGAGGACACTGCTCCTACACCCAGCCATTTTTGGCCTTCATCACATTGGAA
TGCAGATGAGAATAGCTATGTTTAGTTTGATTTATAAGAAGACTTTAAAGCTGTCAAGCCGTGTTCTAGATAAAATAAGTATTGGACAACTTGTTAGTCTCCTTTCCAACAACCTGAACAAATTTGATGAAGGACTTGCATTGGC
ACATTTCGTGTGGATCGCTCCTTTGCAAGTGGCACTCCTCATGGGGCTAATCTGGGAGTTGTTACAGGCGTCTGCCTTCTGTGGACTTGGTTTCCTGATAGTCCTTGCCCTTTTTCAGGCTGGGCTAGGGAGAATGATGATGAAG
TACAGAGATCAGAGAGCTGGGAAGATCAGTGAAAGACTTGTGATTACCTCAGAAATGATTGAAAATATCCAATCTGTTAAGGCATACTGCTGGGAAGAAGCAATGGAAAAAATGATTGAAAACTTAAGACAAACAGAACTGAAAC
TGACTCGGAAGGCAGCCTАTGTGAGATАСТТСААТАGCTCAGCCTTСТТСТТСТСAGGGTTCTTTGTGGTGTTTТТАТСТGTGСTTСССТАТGCACTAATCААAGGAATCAТССТССGGAАААТАТТСАССАССАТСТСАТТСTG
САTTGTTCTGCGCATGGCGGTCACTCGGCAATTTCCOTGGGCTGTAСАААСАТGGТАTGACTCTСТTGGAGCAAТАAАСАAAАТАСAGGATTТСТТАСААААGCAAGAATAТАAGACATTGGAATATAAСТТААСGACTАСAGAA
GTAGTGATGGAGAATGTAАCAGCCTTCTGGGAGGAGGGATTTGGGGAATTATTTGAGAAAGCAAААСАAAАСААТААСААТAGAAAAACTTCТААTGGTGATGACAGCCTCТТСТТCAGTААТТТСТСАСТТСТTGGTAСТССТG
TCCTGAAAGATATTAATTTCAAGATAGAAAGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGAGCAGGCAAGACTTCACTTCTAATGGTGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCACAGTGGAAGAAT
TТСАТТСТGTTCTCAGTTТТССTGGATТАTGCCTGGCАССАТТАААGAAAATATCАТСТТTGGTGTTTCCTАTGATGAATATAGATACAGAAGCGTCATCAAAGCATGCCAАСТAGAAGAGGACATCTCСАAGTTTGCAGAGAAA
GACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCAAGAGCAGTATACAAAGATGCTGATTTGTATTTATTAGACTCTCCTTTTGGATACCTAGATGTTTTAACAGAAAAAG
AAATATTTGAAAGCTGTGTCTGTAAACTGATGGCTAACAAAACTAGGATTTTGGTCACTTCTAAAATGGAACATTTAAAGAAAGCTGACAAAATATTAATTTTGCATGAAGGTAGCAGCTATTTTTATGGGACATTTTCAGAACT
ССААААТСТАCAGCCAGAСТТТAGCTCААAАСТСАТGGGATGTGATТСТТТCGACСААТТТAGTGCAGAAAGAAGAAATTCAAТССТААСТGAGACCTTACAСCGTTTCTCАТTAGAAGGAGATGCTCCTGTCTCCTGGACAGAA
АСАААААААСААТСТТТТАААСAGACTGGAGAGTTTGGGGAAAAAAGGAAGAATTСТАТТСТСААТССААТСААСТСТАТАСGAAAATTTTCСАТTGTGCAAАAGACTCCCТТАСАААТGААTGGCATCGAAGAGGATTCTGATG
AGCCTTTAGAGAGAAGGCTGTCCTTAGTACCAGATTCTGAGCAGGGAGAGGCGATACTGCCTCGCATCAGCGTGATCAGCACTGGCCCCACGCTTCAGGCACGAAGGAGGCAGTCTGTCCTGAACCTGATGACACACTCAGTTAA
ССАAGGTCAGAACATTCAСCGAAAGACААСAGCATCСАСАCGAAAAGTGTCACTGGCCCСТCAGGCAAACTTGAСTGAACTGGATATATATTСАAGAAGGTTАТСТСААGAААСTGGCTTGGAAATAAGTтGAAGAAATTААCGAA
GAAGACTTAAAGGAGTGCTTTTTTGATGATATGGAGAGCATACCAGCAGTGACTACATGGAACACATACCTTCGATATATTACTGTCCACAAGAGCTTAATTTTTGTGCTAATTTGGTGCTTAGTAATTTTTCTGGCAGAGGTGG
СTGCTTСТТTGGTTGTGCTGTGGCTCCTTGGAAACACТССТСТТСАAGACAAAGGGAATAGTACTСАТAGTAGAААТААСАGCТАTGCAGTGАТТАТСАССАGCACCAGTTCGTATTATGTGTTTTACATТТАCGTGGGAGTAGC
CGACACTTTGCTTGCTATGGGATTCTTCAGAGGTCTАССАСТGGTGCATACTCТААТСАСАGTGTCGAAAATTTТАСАССАСААААTGTTACАТТСTGTTCTТСАAGCACCТАTGTCAACССТСААСАСGTTGAAAGCAGGTGGG
АТТСТТААТAGATTCTCCААAGATATAGCAATTTTGGATGACCTTCTGCCTСТТАССАТАТТТGАСТТСАТССАGTTGTTATTААТTGTGATTGGAGCTATAGCAGTTGTCGCAGTTTTAСААСССТАСАТСТТTGTTGCAACAG
TGCCAGTGAТAGTGGCTTТТАТТАТGTTGAGAGCATAТТТССТССАААССТСАСАGCAACTCAAАСААСТGGAAТСTGAAGGCAGGAGTCCAАТТТТСАСТСАТСТTGTTAСАAGCTTAAАAGGACTATGGACACTTCGTGCCTT
CGGACGGCAGCCTTACTTTGAAACTCTGTTCCACAAAGCTCTGAATТТАСАТАСТGCCAACTGGTТСTTGTACCTGTCAACACTGCGCTGGTТССАААТGAGAATAGAAATGATTTTTGTСАТСТТСТТСАТTGCTGTTАССТТС
ATTTCCATTTTAACAACAGGAGAAGGAGAAGGAAGAGTTGGTATTATCCTGACTTTAGCCATGAATATCATGAGTACATTGCAGTGGGCTGTAAACTCCAGCATAGATGTGGATAGCTTGATGCGATCTGTGAGCCGAGTCTTTA
AGTTCATTGACATGCCAACAGAAGGTAААССТАССААGTCAACCAAАССАТАСААGAATGGCCAАСТСТСGAAAGTTATGATTАТTGAGAATТСАСАCGTGAAGAAAGATGACATCTGGCССТСAGGGGGCCAAATGACTGTCAA
AGATCTCACAGCAAAATAСАСAGAAGGTGGAAATGCOАТАТТAGAGAACATTTCCТТСТСААТААGTCCTGGCCAGAGGGTGGGCCTСTTGGGAAGAACTGGATCAGGGAAGAGTACTTTGTTATCAGCTTTTTTGAGAСТАСТG
AACACTGAAGGAGAAATCCAGATCGATGGTGTGTCTTGGGATTCAATAACTTTGCAACAGTGGAGGAAAGCCTTTGGAGTGATACCACAGAAAGTATTTATTTTTTCTGGAACATTTAGAAAAAACTTGGATCCCTATGAACAGT
GGAGTGATCAAGAAATATGGAAAGTTGCAGATGAGGTTGGGCTCAGATCTGTGATAGAACAGTTTCCTGGGAAGCTTGACTTTGTCCTTGTGGATGGGGGCTGTGTCCTAAGCCATGGCCACAAGCAGTTGATGTGCTTGGCTAG
ATCTGTTCTCAGTAAGGCGAAGATCTTGCTGCTTGATGAACCCAGTGCTCATTTGGATCCAGTAACATACCAAATAATTAGAAGAACTCTAAAACAAGCATTTGCTGATTGCACAGTAATTCTCTGTGAACACAGGATAGAAGCA
ATGCTGGAATGCCAACAATTTTTGGTCATAGAAGAGAACAAAGTGCGGCAGTACGATTCCATCCAGAAACTGCTGAACGAGAGGAGCCTCTTCCGGCAAGCCATCAGCCCCTCCGACAGGGTGAAGCTCTTTCCCCACCGGAACT
CAAGCAAGTGCAAGTCTAAGCCCCAGATTGCTGCTCTGAAAGAGGAGACAGAAGAAGAGGTGCAAGATACAAGGCTTTAGAGAGCAGCATAAATGTTGACATGGGACATTTGCTCATGGAATTGGAGCTCGTGGGACAGTCACCT
CATGGAATTGGAGCTCGTGGAACAGTTACCTCTGCCTCAGAAAACAAGGATGAATTAAGTTTTTTTTTAA
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