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Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small number of base pairs randomly somewhere into the gene. They isolate several versions (with different insertions) of this modified gene and carry out an RT-PCR using a primer that recognizes the transcriptional start site area (eg. from +1 to +20), and a primer that binds to the polyA tail. For the second modified gene sample, they observe that the mature mRNA is now several base pairs longer, however splicing has not been affected. They examine the protein and identify that it has significant changes to its amino acid sequence, altering its length. Which of the lettered arrowheads indicates the location where this insertion could be? (There could be one answer or several. Give all answers that apply)

Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small number of base pairs randomly somewhere into the gene. They isolate several versions (with different insertions) of this modified gene and carry out an RT-PCR using a primer that recognizes the transcriptional start site area (eg. from +1 to +20), and a primer that binds to the polyA tail. For the second modified gene sample, they observe that the mature mRNA is now several base pairs longer, however splicing has not been affected. They examine the protein and identify that it has significant changes to its amino acid sequence, altering its length. Which of the lettered arrowheads indicates the location where this insertion could be? (There could be one answer or several. Give all answers that apply)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small
number of base pairs randomly somewhere into the gene. They isolate several versions (with different
insertions) of this modified gene and carry out an RT-PCR using a primer that recognizes the
transcriptional start site area (eg. from +1 to +20), and a primer that binds to the polyA tail.

For the second modified gene sample, they observe that the mature mRNA is now several base
pairs longer, however splicing has not been affected. They examine the protein and identify that
it has significant changes to its amino acid sequence, altering its length. Which of the lettered
arrowheads indicates the location where this insertion could be? (There could be one answer or
several. Give all answers that apply)

Here is a human gene, called cxx2. The following locations are marked: where transcription starts and ends, the start codon and the stop codon, and the boundaries of the two exons and the one intron. The open arrowheads (triangles) indicate specific locations in the gene. Use these locations (1-10) to answer the following questions. 1 2 transcriptional start site +1 3 Ĵ ATG exon/intron intron/exon boundary boundary intron transcriptional stop site 9 8 TAA 10
Transcribed Image Text:Here is a human gene, called cxx2. The following locations are marked: where transcription starts and ends, the start codon and the stop codon, and the boundaries of the two exons and the one intron. The open arrowheads (triangles) indicate specific locations in the gene. Use these locations (1-10) to answer the following questions. 1 2 transcriptional start site +1 3 Ĵ ATG exon/intron intron/exon boundary boundary intron transcriptional stop site 9 8 TAA 10
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