You would like to amplify the gene shown below using PCR with two primers: the primers are shown on each side of the gene but are not yet positioned relative to the sequence. What sequence should primer 2 have? Primer 2 3'GGGCCA--- 5'CCCGGT-.. 3' CCCGGT 5' 5' GGGCCA 3' 3' GGGCCA 5' 5' CCCGGT 3' gene --- TGTACA 5' ----ACATGT 3' Primer 1

Q: The target sequence for a PCR amplification is shown below. When the final cycle of PCR has ended,…

A: The polymerase chain reaction (PCR), often known as "molecular photocopying," is a rapid and…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: vWA produce 200bp in PCR . All others produce less or more than 200 bp in PCR.  Here SSR means is…

Q: Design as a set of primers, every 20 nucleotides in length. Using this sequence as a guide, design a…

A: PCR or polymerase chain reaction is a rapid and versatile in vitro technique for amplification of…

Q: You would like to amplify the gene shown here using PCR.  Assume the primer to the 5' end of the…

A: PCR stands for Polymerase Chain Reaction. This is used to amplify the gene of interest.

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: Following a dye terminator DNA sequencing reaction using 2',3'-dideoxynucleotide triphosphates…

A: Dye terminator DNA sequencing is the modern version of Sanger sequencing. It uses…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 3 reasons.

A: DNA sequences can be amplified in vitro using the PCR (Polymerase Chain Reaction) method. A…

Q: Select two primers from the list below such that your selected primers create a pair capable of…

A: Primer is a short strand of nucleotide sequence which helps in synthesis of DNA. After the…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: What primers will be used to sequence your PCR product where are those sequences on your PCR product…

A: PCR is a technique used for amplification of DNA/gene of interest. At the end of the PCR technique…

Q: Transposon mutagenesis

A: Transposons: These are also known as jumping genes. These are the segments of DNA which can move or…

Q: Which of the following is a correct statement about the primers used in the ALU insertion PCR…

A: Answer -  Option B - The primers for both + and - alleles are the same and are on flanking regions…

Q: you wanted to design two primers to PCR amplify a gene from an organism that has 4 chromosomes, each…

A: In PCR that is, polymerase chain reaction two primers are needed  to hybridize with the sample DNA…

Q: DNA containing the genes gin+ phe+ lys+ was used to transform bacterial cells of genotype gln- phe-…

A: As per the question :-  Genotypes and number of individuals obtained are as:-  A ) gly + phe+ lys +…

Q: Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

A: The term SSR stands for simple sequence repeat that is a microsatellite VNTR (variable number tandem…

Q: The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence…

A: PCR is a polymerase chain reaction, this is a biotechnological process which is used to amplify the…

Q: What is the probability that any random male in USA would share the same genotype as the murderer…

A: SSR (simple sequence repeats) or microsatellite refers to a tract of repetitive co-dominant…

Q: You want to amplify a specific region of DNA during PCR. Your primers should

A: Primeras are short sequences of DNA with up to 18-20 base pairs composed of nitrogenous bases (…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.

A: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences in vitro. The process…

Q: The temperature at which the primers and target DNA hybridize may be changed to influence the…

A: Introduction Temperature changes have an impact on the response, which may be noticed at both high…

Q: Below is the DNA sequence (coding strand) for the human placental protein 14 (not all of the gene…

A: PCR stands for Polymerase Chain Reaction. It's a widely used laboratory technique in molecular…

Q: After running a qPCR experiment, we will have graphs showing the amount of fluorescence detected by…

A: The Polymerase Chain Reaction is a qualitative procedure which is used to produce large copies of…

Q: A possible forward primer to be designed is a primer that binds to the region of the OXA-M290 gene…

A: Cloning is a fundamental technique in molecular biology that involves inserting a gene of interest…

Q: You are studying a genome that has 42% G:C content and the remaining 58% of the genome is As and Ts.…

A: Restriction enzymes  The enzyme that is use to cut the DNA at specific site.

Q: 19. You run a PCR of the above HBB sequence using the primers you designed. You receive a vector…

A: i) Gel Electrophoresis Bands:- The ladder serves as a reference for the sizes of the DNA fragments.-…

Q: After you design the PCR primers, you run the PCR on fluid from the patient’s nasal swab. Next, you…

A: Gel electrophoresis is the widely used technique in molecular biology for the separation of the…

Q: 8. You perform a PCR experiment using the template and primers shown below. What size is the…

A: PCR stands for Polymerase Chain Reaction. It is a laboratory technique used to amplify a single or a…