Transposon mutagenesis

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transposon mutagenesis was used to generate a library of
mutants within the Salmonella genome. You are trying to
identify a colony with the transposon inserted in the
pathogenic related gene SPI-1 using PCR. Forward and
reverse primers are generated that flank either side of the
gene and yield a wild type product that is 900 bases in
length. Which of the colonies sampled in the gel would
you expect to contain the SPI-1 gene with transposon
insertion?
3,000
2,000
1,000
700
500
300
100
Ladder Colony A Colony B Colony C Colony D Colony E
none
colonies A&C
colonies B&E
O colonies A, C, &D
colonies B, D, &E
-
Transcribed Image Text:Transposon mutagenesis was used to generate a library of mutants within the Salmonella genome. You are trying to identify a colony with the transposon inserted in the pathogenic related gene SPI-1 using PCR. Forward and reverse primers are generated that flank either side of the gene and yield a wild type product that is 900 bases in length. Which of the colonies sampled in the gel would you expect to contain the SPI-1 gene with transposon insertion? 3,000 2,000 1,000 700 500 300 100 Ladder Colony A Colony B Colony C Colony D Colony E none colonies A&C colonies B&E O colonies A, C, &D colonies B, D, &E -
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