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Design as a set of primers, every 20 nucleotides in length. Using this sequence as a guide, design a set of PCR primers that would amplify ANY particular 500 bp (exactly) segment of this sequence. You pick what part of the sequence you are interested in. Remember that the sequence shown in the database is the coding strand written 5’ to 3’. Remember that your primer set needs to amplify double-stranded DNA. Your forward primer will bind to the end of one strand, the reverse primer must bind to the other... think this through carefully.  Remember to type your sequence in all CAPITAL letters (GATC) and in indicating the proper polarity (5’and 3’) on each sequence you made above indicating which is the forward and which is the reverse primer.

Design as a set of primers, every 20 nucleotides in length. Using this sequence as a guide, design a set of PCR primers that would amplify ANY particular 500 bp (exactly) segment of this sequence. You pick what part of the sequence you are interested in. Remember that the sequence shown in the database is the coding strand written 5’ to 3’. Remember that your primer set needs to amplify double-stranded DNA. Your forward primer will bind to the end of one strand, the reverse primer must bind to the other... think this through carefully.  Remember to type your sequence in all CAPITAL letters (GATC) and in indicating the proper polarity (5’and 3’) on each sequence you made above indicating which is the forward and which is the reverse primer.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Design as a set of primers, every 20 nucleotides in length. Using this sequence as a guide, design a set of PCR primers that would amplify ANY particular 500 bp (exactly) segment of this sequence. You pick what part of the sequence you are interested in.

    1. Remember that the sequence shown in the database is the coding strand written 5’ to 3’.

    2. Remember that your primer set needs to amplify double-stranded DNA. Your forward primer will bind to the end of one strand, the reverse primer must bind to the other... think this through carefully. 

      Remember to type your sequence in all CAPITAL letters (GATC) and in indicating the proper polarity (5’and 3’) on each sequence you made above indicating which is the forward and which is the reverse primer.

       
CTGCTGGNCCGGGTGCTAGGNCCCTGACTGCCCGGGGCCGGGGGTGCGGGGCCCGCTGAGCCCGCGCCCA ССTGGAACTCGCGCTGGCTGGCGAGCGCTтGCGCGCAGNСССAGTTCCCАСАСССGCCTтСТСССТССАСАС TTCCCCGCAAGCAGAGGGAGCCGGCTCTGGCTTCGGCCAGCCCAGAGAGGGGCCCCCACAGCGCAGTGGC GGGCTGAAGGGCTCCTCCAGCACGGNCAGAATGGACGCCAAGGNCGAGGAGGCGCCGAGAGCGAGCGAGG GCTGCTAGСАCGTTGTCАССТСGCATТСTGAACCACAGACTCTCСААСТСТСОGGNGCTTTТCGCCCAСT
Transcribed Image Text:CTGCTGGNCCGGGTGCTAGGNCCCTGACTGCCCGGGGCCGGGGGTGCGGGGCCCGCTGAGCCCGCGCCCA ССTGGAACTCGCGCTGGCTGGCGAGCGCTтGCGCGCAGNСССAGTTCCCАСАСССGCCTтСТСССТССАСАС TTCCCCGCAAGCAGAGGGAGCCGGCTCTGGCTTCGGCCAGCCCAGAGAGGGGCCCCCACAGCGCAGTGGC GGGCTGAAGGGCTCCTCCAGCACGGNCAGAATGGACGCCAAGGNCGAGGAGGCGCCGAGAGCGAGCGAGG GCTGCTAGСАCGTTGTCАССТСGCATТСTGAACCACAGACTCTCСААСТСТСОGGNGCTTTТCGCCCAСT
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