Specifically, why is the primer needed in order for DNA ploymerase to start synthesis? A ) primers provide the free OH group needed by DNA polymerase to make the first covalent bond B) to prevent DNA helicase from breaking too many H bonds C) the DNA is in danger of becoming denatured without a primer D) primers provide the free phosphate group needed by DNA polymerase to make the first covalent bond E) primers will provide enough energy to help the reaction occur

Q: In pcr experiment, Does electrophoresis show that only DNA products of the desired size are present?…

A: A DNA sequence may be amplified trillions of times via PCR, yielding enough DNA copies for further…

Q: How many PCR cycles are required to generate double-stranded target fragments?

A: PCR (Polymerase Chain reaction) is a chain of three reactions that occurs one after the other. It…

Q: Polymerase chain reaction (PCR) is a technique used to amplify a fragment of DNA. When conducting…

A: PCR is a method used to rapidly make millions of copies of a specific DNA sample and amplify DNA…

Q: compare and contrast DNA replication in the cell with PCR-based replication in the lab. Do these two…

A: DNA replication vs pCR(polymerase chain reaction). *DNA replication is a process that produce two…

Q: You are an expert molecular biologist and you just had your regular PCR run and you are now checking…

A: Following are the steps you will make to be able to avoid this in your next PCR run?   1. do the…

Q: Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of…

A: PCR is a scientific method for amplifying a particular DNA segment from just a small portion of…

Q: Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the…

A: Introduction  When the DNA chromosome is replicated, two copies of each identical daughter cell are…

Q: Your graduate advisor asks you to amplify the following sequence of DNA by PCR:…

A: The primer should be complementary to the region of template to be amplified.

Q: During a PCR reaction, the step in the cycle where dNTPs are added to the primer 3’ ends to fill in…

A: Kary Mullis created the groundbreaking technique known as PCR (Polymerase Chain Reaction) in the…

Q: PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3'…

A: The PCR is known as a polymerase chain reaction in which the DNA template is amplified with the help…

Q: An investigator obtains a bacterial temperature-sensitive mutation that affects a step in the…

A: Every DNA molecule is twisted into a ladder form known as a "double helix." The backbone of the DNA…

Q: PCR uses two short DNA sequences (the primers) that bind to the two DNA strands of the original…

A: DNA replication is a complex process of producing copies of the entire chromosome using the…

Q: A PCR reaction was performed to amplify the CHE2 gene. The PCR primers used were designed to amplify…

A: Polymerase chain reaction is a molecular biology technique that is sued to synthesize multiple…

Q: DNA polymerases have exonuclease activity that hydrolyzes DNA in the 3′ to 5′ direction. When an…

A: DNA POLYMERASE: DNA polymerase is an enzyme that plays a key role in the replication of DNA, the…

Q: the RNA primer at the beginning of each Okazaki fragment is removed by : A-DNA polymerase I  B-DNA…

A: Replication Replication is defined as the process of synthesis of new DNA from the existing DNA…

Q: You want to clone a specific PCR amplicon. You have determined that the amplicon you want to clone…

A: Restriction enzymes are endonucleases derived from bacterial cells that cleave DNA (deoxyribonucleic…

Q: Order the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of…

A: Step 1: The order of the steps required to sequence a region of DNA using dideoxy sequencing…

Q: The original DNA template starts with T following the primer sequence if the smallest fragment…

A: Dideoxy nucleotides are similar to regular, or deoxy, nucleotides, but they lack a  3’ OH group.…

Q: Which of the following is/are true of DNA general recombination? The process involves invasion of a…

A: DNA recombination in general is a natural process that occurs in a wide range of organisms,…

Q: Components/Steps DNA gyrase/Helicase Primase RNA primer Purpose/function of components/steps in…

A: PCR is the process of copying DNA in-vitro or outside the cell. It mimics the process of DNA…

Q: Examine the DNA sequence shown below. You have been tasked with designing Primers for PCR…

A: Polymerase chain reaction (PCR) is a method used to  make millions of copies of a specific DNA…

Q: Examine the sequence for the DNA fragment below. Your job is to design primers for PCR that would be…

A: A primer may be a brief single-stranded nucleic acid utilized by all living living beings within the…

Q: Phosphorus is required to synthesize the deoxyribonucleoside triphosphates used in DNA replication.…

A: DNA replication is a semi-conservative process in which newly synthesized double helix DNA consists…

Q: The PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There…

A: PCR is a sensitive technique which helps in making rapid amplification of a specific DNA segment.…

Q: Once separated from other cellular components, extracted DNA can be used for a variety of purposes.…

A: Introduction:- Biotechnology relies heavily on the extraction of DNA. It serves as the foundation…

Q: How do the enzymes involved in the mismatch repair process determine which of the two bases in the…

A: DNA polymerases are the enzymes that build DNA in cells. During DNA replication (copying), most DNA…

Q: Why do DNA Polymerases Require a Primer?

A: DNA Polymerase DNA Polymerase is the enzyme responsible for normal DNA synthesis. Different kinds of…

Q: A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease.…

A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…

Q: You have two PCR primers: Forward- 5' TGAGCTAGGC 3' and Reverse- 5' GGTTCAGTCAG 3'. Show the binding…

A:

Q: (A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the…

A: PCR stands for the polymerase chain reaction, which is used to generate larger copies of the gene of…

Q: complimentary sticky ends

A: DNA's are cut by using restriction enzymes ( endonuclease or exonuclease) which produce sticky or…

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A: Addition of TTGGGG repeats Correct match for this statement are - Telomerase.   Explanation…

Q: For PCR, what property of DNA does temperature influence? Why do the “denaturation” and “annealing”…

A: PCR stands for polymerase chain reaction. It is an in-vitro laboratory technique used for generating…

Q: The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers…

A: PCR or polymerase chain reaction is a rapid and versatile in vitro technique for amplification of…

Q: Explain why DNA ladders are usually included during gel electrophoresis.

A: BASIC INFORMATION  GEL ELECTROPHORESIS  It is a process in which the DNA fragments are separated…

Q: Figure 2 illustrates the important elements of a cosmid. a) Briefly describe the importance of…

A: In the recombinant DNA technology the foreign DNA or target DNA is inserted into the vector then the…

Q: To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on…

A: Specific sequences can be amplified by PCR in order to generate large amounts of DNA to manufacture…

Q: Most PCR reactions do not use the more expensive types of DNA polymerase, which have DNA…

A: DNA polymerases inside the cell, especially the polymerases I, II, and III, have proof reading…

Q: To be able to properly analyze a sample of DNA, you need to have at least 1 µg of the DNA of…

A: The polymerase chain reaction (PCR) is a molecular biology technique for amplifying particular…

Q: Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive…

A: PCR stands for polymerase chain reaction. PCR is used to amplify nucleic acid segments for further…

Q: A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the…

A: SYBR TAQMAN Not so costly Expensive Easy to use Difficult to use Binding the fluorescent dye…

Q: An investigator obtalns a bacterial temperature-sensitive mutation that affects a step in the…

A: DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.