An investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that Okazaki fragments accumulate in unusually large quantities.  What is the likely target affected by the temperature-sensitive mutation?   primase DNA polymerase III DNA helicase DNA Ligase DNA gyrase

Q: Choose the primary impact of the given agents on the genetic material. Choices: deletion of…

A: Any kind of alteration in genetic material which is permanent in nature called Mutation. Two types…

Q: he Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication…

A: The objective of the question is to understand the expected results of the Meselson and Stahl…

Q: Please explain one type of “DNA repair” mechanism (what is it, when is it used, how does it work).

A: The process by which a cell detects and repairs damage to the DNA molecules that encode its genome…

Q: Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain…

A: Polymerase (PCR) chain reaction is a process, during which the specific DNA segment is copied in a…

Q: Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the…

A: Introduction  When the DNA chromosome is replicated, two copies of each identical daughter cell are…

Q: Chromosomal translocations AND mutations in cadherin binding sites can result in cancer. Select one:…

A: Introduction: When a segment of one chromosome separates and joins another chromosome, the process…

Q: Sometimes DNA polymerase makes a mistake, and the wrong nucleotide is added to the growing DNA…

A: In the realm of genetic mutations, scientists have observed a notable discrepancy between two…

Q: Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a…

A: DNA polymerase is an important enzyme of dna replication and can add nucleotide triphosphates to 3’…

Q: Which of the following enzymes at the bacterial replication fork hydrolyze ATP for their activity?…

A: Introduction :- DNA primase, a type of RNA polymerase, is an enzyme that aids in DNA replication. A…

Q: Which of the following enzymes has 5’ to 3’ exonuclease activity? DNA ligase DNA polymerase I…

A: To shift DNA strands, the 5′ to 3′ exonuclease activity can be used with the polymerization…

Q: Choose the primary impact of the given agents on the genetic material. Choices: deletion of…

A: DNA replication, transcription or translation are the targets of the natural or synthetic…

Q: Each set consist of five key players in the process involved in the central dogma. Choose ONLY two…

A: DNA in a cell undergoes replication process to duplicate the DNA and transfers the duplicated DNA…

Q: In bacterial cells, nucleotide excision repair involves which of the following proteins? DNA…

A: The principal method utilised by mammals to remove bulky DNA lesions such as those caused by UV…

Q: In light of your knowledge of DNA replication, discuss the following statement: "Only the DNA is…

A: Dna replicates semiconservatively is proven by matthew meselson and franklin stahl by performing an…

Q: 1 MSH2 MSH6 Template strand 5' A 3' Newly synthesized daughter strand MLH1 endonuclease, PMS2 2 DNA…

A: The mismatch excision-repair pathway corrects errors introduced during replication Step 1: A complex…

Q: Specifically, why is the primer needed in order for DNA ploymerase to start synthesis? A ) primers…

A: Always replication, Transcription starts from 5' to 3'. Addition of nucleotide takes place at 3'-OH.

Q: Which of the DNA polymerases shown in Table  have the ability to proofread?

A: The DNA polymerase enzyme uses a single stranded DNA molecule as a template and causes the synthesis…

Q: The general excision repair pathway for DNA repair has the following order   A. endonuclease cuts…

A: When there is an exposure of any mutagens either UV radiation or chemicals, there is the occurrence…

Q: there is nucleotide selectivity during replication, why do polymerase choose a wrong nucletide and…

A: A nucleotide is the basic building block of nucleic acids, such as DNA and RNA. It is composed of…

Q: the RNA primer at the beginning of each Okazaki fragment is removed by : A-DNA polymerase I  B-DNA…

A: Replication Replication is defined as the process of synthesis of new DNA from the existing DNA…

Q: 1). DNA polymerase III is the bacterial replicative polymerase. It is comprised of subunits derived…

A: DNA polymerase III transcribes the genetic code in bacteria. Polθ and Poleε subunits function as…

Q: Reiji and Tuneko Okazaki conducted a now classic experiment in1968 in which they discovered a…

A: The DNA (deoxyribonucleic acid) replication occurs in a semi-discontinuous manner. In this process,…

Q: Choose the primary impact of the given agents to the genetic material. choices: arrrests DNA…

A: The given list of agents are those that interfere with DNA replication with different specificities…

Q: can someone explain the picture in simple terms? how does the parp inhibitor work

A: PARP or Poly ADP Ribose Polymerase is a proteinaceous enzyme responsible for repairing damaged DNA…

Q: Which of the following is/are true of DNA general recombination? The process involves invasion of a…

A: DNA recombination in general is a natural process that occurs in a wide range of organisms,…

Q: Which of the following has 5’ to 3’ exonuclease activity? DNA polymerase I DNA polymerase III DNA…

A: The 5'-3' exonuclease activity's primary objective is to eliminate the RNA primers from newly…

Q: Considering prokaryotes, what is the enzyme that helps hold DNA polymerase III in place when…

A: In prokaryotes, replication is a process by which it makes identical copies of DNA. The process…

Q: With regard to DNA replication, define the term bidirectional replication.

A: Deoxyribonucleic acid (DNA) is a double-stranded molecule, which consists of two strands of…

Q: DNA polymerase III (the main polymerase) Helicase DNA ligase Single-stranded binding proteins DNA…

A: DNA replication is the process by which new DNA strands are produced from the old DNA strands by the…

Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…

A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…

Q: Place the steps of base excision repair in order. Not all choices will be used. hydrolyze base First…

A: In base excision repair the incorrect bases are recognised and remove from the DNA. In this…

Q: Phosphorus is required to synthesize the deoxyribonucleoside triphosphates used in DNA replication.…

A: DNA replication is a semi-conservative process in which newly synthesized double helix DNA consists…

Q: Assume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single…

A: The objective of the question is to calculate the number of copies of a single DNA fragment after…

Q: Which of the following will lead to DNA cutting more frequently? Choose all that apply.

A: The restriction enzymes are enzymes that cut the DNA at specific nucleotide sequences called the…

Q: Why do DNA Polymerases Require a Primer?

A: DNA Polymerase DNA Polymerase is the enzyme responsible for normal DNA synthesis. Different kinds of…

Q: Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA…

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around…

Q: What is the name of the process of rehybridization of melted DNA? replication denaturation annealing…

A: DNA hybridization is the process, in which two strands of the complementary DNA are joined to form…

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A:

Q: Spontaneous oxidative deamination of guanine produces the base xanthine (see figure, which also…

A: As per the Watson-Crick model of the DNA double helix:DNA is made up of two strands of…

Q: The template (antisense) strands of two complete (double-stranded) DNA molecules have the base…

A: Semiconservative replicationMatthew Meselson and Franklin Stahl provided the experiment which shows…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.