TABLE Characteristics of DNA polymerases in E. coli 3' -5' Polymerase Exonuclease Exonuclease Activity 5' - 3' 5'-3' DNA Polymerase Activity Activity Function Removes and replaces primers Yes Yes Yes II DNA repair; restarts replication after damaged DNA halts synthesis Yes Yes No III Yes Yes No Elongates DNA IV Yes No No DNA repair Yes No No DNA repair; translesion DNA synthesis

Q: How  Polymerase Chain Reaction (PCR)Amplifies Defined Regions of a Genome?

A: Polymerase chain reaction or PCR technique is used to amplify or make multiple copies of a specific…

Q: List and describe three important processes are affected by DNA supercoilin

A: DNA supercoiling is reffered to as under or "overwinding' of DNA strands. It is a crucial process in…

Q: What is the main reason for using a cDNA library rather than a genomic library to isolate a human…

A: Introduction :- cDNA stands for "complementary DNA". It is a type of DNA that is synthesized from…

Q: Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain…

A: Polymerase (PCR) chain reaction is a process, during which the specific DNA segment is copied in a…

Q: Assuming that you start with one genome copy and are trying to amplify a 2000 bp DNA fragment, how…

A: PCR (Polymerase Chain Reaction) is a laboratory technique for amplifying a specific DNA segment. The…

Q: What are the five main steps in DNA cut-and-paste transposition?

A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…

Q: Can you explain what semiconservative means in relationship to the way DNA is copied?

A: In the process of DNA replication, two daughter DNAs are formed with one old and one new strand…

Q: Design as a set of primers, every 20 nucleotides in length. Using this sequence as a guide, design a…

A: PCR or polymerase chain reaction is a rapid and versatile in vitro technique for amplification of…

Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…

A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…

Q: If you wanted to study the nature of transcription in yeast under aerobic versus anaerobic…

A: Yeast grows differently under aerobic and anaerobic conditions. To study the nature of transcription…

Q: What is a repetitive element in genomics?  What are the types of repetitive elements?  What is their…

A: The amount  of DNA present in a cell of a person is called as genome. DNA is made up of nucleotides…

Q: The polymerase chain reaction can only be used to amplify genes that have been cloned and sequenced.…

A: The polymerase chain reaction was originally developed by the American biochemist Kary Mullis. The…

Q: Describe an experimental approach to determining the processivity of a DNA polymerase (that is, the…

A: The processivity is defined as the degree of the addition of an average number of nucleotides prior…

Q: Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by…

A: Synthetic probe is fragment of DNA. to screen a DNA library RNA of variable length approximately 100…

Q: Describe a high-throughput, automated method bywhich millions of DNA templates may be…

A: The process of determining the exact order in which the sequence of the nucleotides are present in…

Q: Does proofreading always take place by the same process in replication?

A: REPAIR PATHWAYS:- Both in Prokaryotes and Eukaryotes, there is a repair enzyme system to deal with…

Q: Can you explain what is nucleotide selectivity in polymerase

A: One of the structural elements, or building blocks, of DNA and RNA is the nucleotide. A nucleotide…

Q: does human contain supercoiling DNA？Why supercoiling is important and what is it？

A: A higher-order DNA structure is described by DNA supercoiling. Two complimentary strands are wound…

Q: The major products of proofreading by RNA polymerase are dinucleotides rather than mononucleotides.…

A: Introduction: DNA polymerase holds the ability to proofread the strand synthesized during…

Q: Mention all the enzymes and reagents used in the everse Transcriptase Polymerase Chain Reaction…

A: RT PCR  is Reverse transcription polymerase chain reaction. This a process used to generally…

Q: what are some Limitations/sources of error with suitable rationalization that may occur during the…

A: PCR is in vitro method for amplifying defined target DNA sequences present within the source of DNA.…

Q: Explain why genomic DNA libraries require more colonies than are comprised by a single genome…

A: Genes are the units of heredity that are transmitted through generations. Genes contain the genetic…

Q: What are the main  differences between whole genome sequencing and whole exome sequencing?

A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…

Q: Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain…

A: The polymerase chain reaction (PCR) is the process of artificially synthesizing multiple copies of a…

Q: What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA…

A: Your question contains multiple sub-parts, and as per our policy, we only answer the first three…

Q: Earlier, proofreading DNA polymerases used to have higher fidelity than that of Taq DNA pol. but…

A: PCR is polymerase chain reaction. It was used initially for polymerization of the small fragments of…

Q: Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the EcoRI…

A: No,  because most humans genes are much longer than 4kb.

Q: What type of repair would be used to fix a mutation that arises during replication and that escapes…

A: DNA replication is the type of DNA mechanism in which the copies of DNA are made. During the process…