Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a detailed explanation.

Q: Suppose that you wish to make a sample of DNA duplex highly radioactive to use as a DNA probe. You…

A: Ionizing radiation direct affects DNA structure by inducing DNA breaks, individually, DSBs.…

Q: Does Phospodiester bonds hold deoxyribose and phosphate components together in the DNA

A: Ans- true

Q: How is the DNA unwound at the replication fork? What effect does this have on the DNA upstream of…

A: DNA It is a double stranded helical structure present in almost all eukaryotic cell.

Q: The enzymatic activity of the DNase I variants was tested using a DNA hyperchromicity assay. The…

A: Enzymes are proteinaceous entities responsible for carrying out as well as boosting the overall rate…

Q: What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase

A: Enzymes serve as biological catalysts that fasten the rate of reaction by lowering the activation…

Q: Provide a detailed description with a hand-drawn figure of the role DNA recombination plays in…

A: Double-stranded breaks (DSB) occur frequently in eukaryotic cells. It can be caused by many factors…

Q: List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish…

A: Introduction  DNA pol III is the primary enzyme which leads the DNA replication system. It is a…

Q: Explain the process of DNA elongation. In your discussion, make sure you discuss the direction of…

A: Basically, elongation is the stage when the RNA strand gets longer, thanks to the addition of new…

Q: There are various types of DNA-targeting drug, including DNA alkylating agents, DNA intercalators…

A: Mutations are DNA errors that occur during life. A mutation is defined as any alteration in the DNA…

Q: What are the requirements for strand elongation by DNA polymerase enzymes?

A: DNA replication in eukaryotes occurs in three stages: initiation, elongation, and termination,…

Q: Describe an experimental approach to determining the processivity of a DNA polymerase (i.e., the…

A: Processivity is the average number of bases a polymerase enzyme will extend and incorporate base…

Q: Why does a fiber ( double stranded molecule or duplex) of DNA shorten upon drying?

A: DNA The double helical structure present inside the nucleus.

Q: Explain the mechanism of trinucleotide repeat expansion.

A: Introduction: A trinucleotide repeat expansion is type of DNA mutation. In which, the trinucleotide…

Q: In experiments using polymerase chain reactions (PCR), it is often more difficult to amplify through…

A: Introduction :- Polymerase Chain Reaction (PCR) is a laboratory technique used to make many copies…

Q: DNA polymerase requires both a template, to be copied, and a primer, which provides a 3′ hydroxyl…

A: DNA polymerase is a main enzyme for DNA replication.. The two main requirements for working of  DNA…

Q: Show the watson-crick model for semoconservative DNA replication?

A: We know that, DNA is the largest macro molecule that represents the genetic material of the cell.…

Q: Explain why it is necessary that DNA is replicated continuously on the leading strand by but…

A: Introduction :  Direction of DNA replication is 5' - 3' .  Primase add primer and DNA polymerase…

Q: Cytosine

A: Introduction :- A mutation occurs when a DNA gene is damaged or changed in such a way as to alter…