An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but
not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double-
stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation?
DNA Ligase
DNA polymerase lII
primase
DNA gyrase
Single-stranded DNA-binding protein (SSB)
Transcribed Image Text:An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation? DNA Ligase DNA polymerase lII primase DNA gyrase Single-stranded DNA-binding protein (SSB)
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