What causes the change in the ability of DNA to absorb UV light when it is denatured?
options:
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In denatured DNA, DNA double helix is disrupted, which causes the exposure of the deoxyribose and thus increases their absorbance of UV light |
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In denatured DNA, DNA double helix is disrupted, which causes the exposure of the phosphate groups and thus increases their absorbance of UV light. |
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In denatured DNA, DNA double helix is disrupted, which causes the exposure of the bases and thus increases their absorbance of UV light. |
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all of the above are correct |
The correct option is the C option: In denatured DNA, DNA double helix is disrupted, which causes the exposure of the bases and thus increases their absorbance of UV light.
When DNA is denatured, the ability of the DNA to absorb UV light increased due to the heat the base becomes unstacked. as the base unstacked the maximum absorbance wavelength will not change but the amount absorbed increased by 35% approximately.
the absorbance is increased because the base is exposed outside. clearly note that the absorbance is due to the ring structure of nitrogenous bases.
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