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Some E. coli mutants contain defective DNA ligase. When researchers expose these mutants to H-labeled thymine and then sediment the DNA produced on an alkaline sucrose density gradient, two radioactive bands appear. One corresponds to a high molecular weight fraction, the other to a low molecular weight fraction. Select the reason for the appearance of two radioactive bands. The high molecular weight fraction is the leading strand, whereas the low molecular weight fraction is the completed but unjoined Okazaki fragments from the lagging strand. The high molecular weight fraction is the parent DNA, whereas the low molecular weight fraction is the newly synthesized DNA. The high molecular weight fraction is the newly synthesized DNA, whereas the low molecular weight fraction is the parent DNA. The high molecular weight fraction is the lagging strand, whereas the low molecular weight fraction is the fragmented leading strand that DNA polymerase could not fully synthesize.

Some E. coli mutants contain defective DNA ligase. When researchers expose these mutants to H-labeled thymine and then sediment the DNA produced on an alkaline sucrose density gradient, two radioactive bands appear. One corresponds to a high molecular weight fraction, the other to a low molecular weight fraction. Select the reason for the appearance of two radioactive bands. The high molecular weight fraction is the leading strand, whereas the low molecular weight fraction is the completed but unjoined Okazaki fragments from the lagging strand. The high molecular weight fraction is the parent DNA, whereas the low molecular weight fraction is the newly synthesized DNA. The high molecular weight fraction is the newly synthesized DNA, whereas the low molecular weight fraction is the parent DNA. The high molecular weight fraction is the lagging strand, whereas the low molecular weight fraction is the fragmented leading strand that DNA polymerase could not fully synthesize.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Some E. coli mutants contain defective DNA ligase. When resesarchers expose these mutants to H-labeled thymine and then sediment the DNA produced on an alkaline sucrose density gradient, two radioactive bands appear. One corresponds to a high molecular weight fraction, the other to a low molecular weight fraction. Select the reason for the appearance of two radioactive bands. The high molecular weight fraction is the leading strand, whereas the low molecular weight fraction is the completed but unjoined Okazaki fragments from the lagging strand. The high molecular weight fraction is the parent DNA, whereas the low molecular weight fraction is the newly synthesized DNA. O The high molecular weight fraction is the newly synthesized DNA, whereas the low molecular weight fraction is the parent DNA. The high molecular weight fraction is the lagging strand, whereas the low molecular weight fraction is the fragmented leading strand that DNA polymerase could not fully synthesize.
Transcribed Image Text:Some E. coli mutants contain defective DNA ligase. When resesarchers expose these mutants to H-labeled thymine and then sediment the DNA produced on an alkaline sucrose density gradient, two radioactive bands appear. One corresponds to a high molecular weight fraction, the other to a low molecular weight fraction. Select the reason for the appearance of two radioactive bands. The high molecular weight fraction is the leading strand, whereas the low molecular weight fraction is the completed but unjoined Okazaki fragments from the lagging strand. The high molecular weight fraction is the parent DNA, whereas the low molecular weight fraction is the newly synthesized DNA. O The high molecular weight fraction is the newly synthesized DNA, whereas the low molecular weight fraction is the parent DNA. The high molecular weight fraction is the lagging strand, whereas the low molecular weight fraction is the fragmented leading strand that DNA polymerase could not fully synthesize.
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