A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label. "5'- 3' 3'-OH ATTACGCAAGGACATTAGAC---5 She reacted a sample of the DNA with DNA polymerase and four different nucleotide mixtures (in an appropriate buffer). Some of the mixtures included dideoxynucleotides (ddNTPs) in relatively small amounts. The reseacher then separated the resulting DNA by electrophoresis on a polyacrylamide gel and located the fluorescent bands on the gel. The image of the gel shows the band patterns that resulted from each of the four nucleotide mixtures. Electrophoresis The table shows the nucleotide mixture for Lane 1. Complete the table by showing the nucleotides or mixutres that could have been used to yield this result. Some of the nucleotides or mixtures may not be used. The table shows the nucleotide mixture for Lane 1. Complete the table by showing the nucleotides or mixutres that could have been used to yield this result. Some of the nucleotides or mixtures may not be used. Lane Nucleotide mixtures dATP, dTTP, dCTP, dGTP, ddTTP dATP, dTTP, dCTP. JGTP, ddGTP dATP, dTTP, dCTP, JGTP, ddCTP ATP PPP deIP doIP AIP dATP, dCTP, dGTP. ddTTP dATP, JTTP, dCTP, dGTP Answer Bank JATP. JTTP, JCTP, JGTP. ddGTP ddTTP dATP. JTTP, JCTP. JGTP JATP, JTTP. JCTP, GTP, ddCTP dATP, dTTP, dCTP, dGTP, ddATP dATP. dCTP. dGTP ddTTP
A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label. "5'- 3' 3'-OH ATTACGCAAGGACATTAGAC---5 She reacted a sample of the DNA with DNA polymerase and four different nucleotide mixtures (in an appropriate buffer). Some of the mixtures included dideoxynucleotides (ddNTPs) in relatively small amounts. The reseacher then separated the resulting DNA by electrophoresis on a polyacrylamide gel and located the fluorescent bands on the gel. The image of the gel shows the band patterns that resulted from each of the four nucleotide mixtures. Electrophoresis The table shows the nucleotide mixture for Lane 1. Complete the table by showing the nucleotides or mixutres that could have been used to yield this result. Some of the nucleotides or mixtures may not be used. The table shows the nucleotide mixture for Lane 1. Complete the table by showing the nucleotides or mixutres that could have been used to yield this result. Some of the nucleotides or mixtures may not be used. Lane Nucleotide mixtures dATP, dTTP, dCTP, dGTP, ddTTP dATP, dTTP, dCTP. JGTP, ddGTP dATP, dTTP, dCTP, JGTP, ddCTP ATP PPP deIP doIP AIP dATP, dCTP, dGTP. ddTTP dATP, JTTP, dCTP, dGTP Answer Bank JATP. JTTP, JCTP, JGTP. ddGTP ddTTP dATP. JTTP, JCTP. JGTP JATP, JTTP. JCTP, GTP, ddCTP dATP, dTTP, dCTP, dGTP, ddATP dATP. dCTP. dGTP ddTTP
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Transcribed Image Text:A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label.
"5'-
3'
3'-OH
ATTACGCAAGGACATTAGAC---5
She reacted a sample of the DNA with DNA polymerase and four different nucleotide mixtures (in an appropriate buffer). Some
of the mixtures included dideoxynucleotides (ddNTPs) in relatively small amounts.
The reseacher then separated the resulting DNA by electrophoresis on a polyacrylamide gel and located the fluorescent bands on
the gel. The image of the gel shows the band patterns that resulted from each of the four nucleotide mixtures.
Electrophoresis
The table shows the nucleotide mixture for Lane 1. Complete the table by showing the nucleotides or mixutres that could have
been used to yield this result. Some of the nucleotides or mixtures may not be used.
The table shows the nucleotide mixture for Lane 1. Complete the table by showing the nucleotides or mixutres that could have
been used to yield this result. Some of the nucleotides or mixtures may not be used.
Lane
Nucleotide mixtures
dATP, dTTP, dCTP, dGTP, ddTTP
dATP, dTTP, dCTP. JGTP, ddGTP
dATP, dTTP, dCTP, JGTP, ddCTP
ATP PPP deIP doIP AIP
dATP, dCTP, dGTP. ddTTP
dATP, JTTP, dCTP, dGTP
Answer Bank
JATP. JTTP, JCTP, JGTP. ddGTP
ddTTP
dATP. JTTP, JCTP. JGTP
JATP, JTTP. JCTP, GTP, ddCTP
dATP, dTTP, dCTP, dGTP, ddATP
dATP. dCTP. dGTP ddTTP
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