Phosphorus is required to synthesize the deoxyribonucleoside triphosphates used in DNA replication. A geneticist grows some E. coli in a medium containing nonradioactive phosphorus for many generations. A sample of the bacteria is then transferred to a medium that contains a radioactive isotope of phosphorus (32P). Samples of the bacteria are removed immediately after the transfer and after one and two rounds of replication. Assume that newly synthesized DNA contains 32P and the original DNA contains nonradioactive phosphorus. What will be the distribution of radioactivity in the DNA of the bacteria in each sample? Will radioactivity be detected in neither, one, or both strands of the DNA?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Phosphorus is required to synthesize the deoxyribonucleoside
triphosphates used in DNA replication. A geneticist grows some E. coli
in a medium containing nonradioactive phosphorus for many
generations. A sample of the bacteria is then transferred to a medium
that contains a radioactive isotope of phosphorus (32P). Samples of the
bacteria are removed immediately after the transfer and after one and
two rounds of replication. Assume that newly synthesized DNA contains
32P and the original DNA contains nonradioactive phosphorus. What
will be the distribution of radioactivity in the DNA of the bacteria in
each sample? Will radioactivity be detected in neither, one, or both
strands of the DNA?

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