The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers hybridized at positions flanking the target region. The arrowheads point in the direction of DNA replication, 5’ to 3’. In the figure and table below, the solid line represents DNA from one strand (call it the "top" strand); the dashed line represents DNA of the complementary strand (the "bottom" strand). The number of PCR products double after each cycle of PCR (Ifn=number of cycles and P= number of product molecules, then P= 2"). The double strand DNA (dsDNA) PCR products fall into a number types; after a number of PCR cycles the target-region dsDNA type becomes the most common product. However, in very early cycles other products can be found represented in appreciable numbers. 5´- 5' Double stranded DNA PCR product # after 2 # after 3 # after 4 # after 15 PCR cycles PCR cycles PCR cycles PCR cycles All types of PCR products FIn the table above, enter the number of duplex DNA products of the illustrated type that will be present among the PCR products after a specified number of cycles. For the images in the table, the positions of the ends of the DNA strands are aligned, relative to each other and to the initial figure above. The dotted lines dropping down from the figure shows the alignments. Assume the PCR reaction was started with a single molecule of double stranded template DNA.
The following diagram shows the two strands of a single DNA molecule, with a pair of PCR primers hybridized at positions flanking the target region. The arrowheads point in the direction of DNA replication, 5’ to 3’. In the figure and table below, the solid line represents DNA from one strand (call it the "top" strand); the dashed line represents DNA of the complementary strand (the "bottom" strand). The number of PCR products double after each cycle of PCR (Ifn=number of cycles and P= number of product molecules, then P= 2"). The double strand DNA (dsDNA) PCR products fall into a number types; after a number of PCR cycles the target-region dsDNA type becomes the most common product. However, in very early cycles other products can be found represented in appreciable numbers. 5´- 5' Double stranded DNA PCR product # after 2 # after 3 # after 4 # after 15 PCR cycles PCR cycles PCR cycles PCR cycles All types of PCR products FIn the table above, enter the number of duplex DNA products of the illustrated type that will be present among the PCR products after a specified number of cycles. For the images in the table, the positions of the ends of the DNA strands are aligned, relative to each other and to the initial figure above. The dotted lines dropping down from the figure shows the alignments. Assume the PCR reaction was started with a single molecule of double stranded template DNA.
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Transcribed Image Text:The diagram illustrates two strands of a single DNA molecule with paired PCR primers hybridized at positions flanking the target region. Arrowheads indicate the direction of DNA replication, from 5’ to 3’. In this illustration, the solid line denotes DNA from one strand ("top"), and the dashed line denotes the complementary strand ("bottom").
The number of PCR products doubles with each PCR cycle (where n is the number of cycles and P is the number of product molecules, P = 2^n). Double-stranded DNA (dsDNA) PCR products will vary in type, but as cycles progress, the dsDNA type of the target region becomes more prevalent. Early cycles may produce other observable products.
**Graph/Diagram Description:**
- A horizontal line represents the DNA strands with arrowheads showing the 5’ to 3’ replication direction.
- Dashed vertical lines indicate alignment of DNA strands.
**Table Explanation:**
- The table lists double-stranded DNA PCR products, showing growth across cycles: 2, 3, 4, and 15.
- Below the table, users are instructed to enter the number of duplex DNA products observed after each cycle, assuming the reaction started with one molecule of double-stranded template DNA.
Assumptions are based on the provided alignment and initial figure.
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