Entire sequence below needs to be amplified by PCR and subcloned into a plasmid vector. Which of the primer sequences listed underneath is the correct reverse primer (6 marks)? Copy correct sequence into your answer. Why primer e) is not the right answer (4 marks)? 5'ATCTCTATTTAATATTTATGTCTATTTAAGCCTCATATTTAAAGACAGGGAAGAGCAGAA CGGAGCCCCAGGCCTCTGTGTCCTTCCCTGCATTTCTGAGTTTCATTCTCCTGCCTGTAGCA GTGAGAAAAAGCTCCTGTCCTCCCATCCCCTGGACTGGGAGGTAGATAGGTAAATACCAAGT ATTTATTACTATGACTGCTCCCCAGCCCTGGCTCTGCAATGGGCACTGGGATGAGCCGCTGT GAGCCCCTGGTCCTGAGGGTCCCCACCTGGGACCCTTGAGAGTATCAGGTCTCCCACGTGGG AGACAAGAAATCCCTGTTTAATATTTAAACAGCAGTGTTCCCCATCTGGGTCCTTGCACCCC TCACTCTGGCCTCAGCCGACTGCACAGCGGCCCCTGCATCCCCTTGGCTGTGAGGCCCCTGG ACAAGCAGAGGTGGCCAGAGCTGGGAGGCATGGCCCTGGGGTCCCACGAATTTGCTGGGGAA TCTCGTTTTTCTTCTTAAGACTTTTGGGACATGGTTTGACTCCCGAACATCACCGACGCGTC TCCTGCTG 3' a) 5' TTCCGGAAGAAGCTTATACGG 3' b) 5' CTGTGTTCACCTAATATTCCT 3' c) 5' CAGCAGGAGACGCGTCGGTGA 3' d) 5' AGGAATATTAGTATAATCCAC 3' e) 5' GACGCGTCGGTGATGTTCGGG 3’ f) 5' ATCTCTATTTAATATTTATGT 3' Answer (word limit 250 words max): Question 2 (10 marks, 250 words max) Discuss factors that might influence the activity of DNA-binding proteins (transcription factors) and bring about changes in gene expression (8 marks). Include in your answer
Entire sequence below needs to be
amplified by PCR and subcloned into a plasmid vector. Which of the primer
sequences listed underneath is the correct reverse primer (6 marks)? Copy correct
sequence into your answer. Why primer e) is not the right answer (4 marks)?
5'ATCTCTATTTAATATTTATGTCTATTTAAGCCTCATATTTAAAGACAGGGAAGAGCAGAA
CGGAGCCCCAGGCCTCTGTGTCCTTCCCTGCATTTCTGAGTTTCATTCTCCTGCCTGTAGCA
GTGAGAAAAAGCTCCTGTCCTCCCATCCCCTGGACTGGGAGGTAGATAGGTAAATACCAAGT
ATTTATTACTATGACTGCTCCCCAGCCCTGGCTCTGCAATGGGCACTGGGATGAGCCGCTGT
GAGCCCCTGGTCCTGAGGGTCCCCACCTGGGACCCTTGAGAGTATCAGGTCTCCCACGTGGG
AGACAAGAAATCCCTGTTTAATATTTAAACAGCAGTGTTCCCCATCTGGGTCCTTGCACCCC
TCACTCTGGCCTCAGCCGACTGCACAGCGGCCCCTGCATCCCCTTGGCTGTGAGGCCCCTGG
ACAAGCAGAGGTGGCCAGAGCTGGGAGGCATGGCCCTGGGGTCCCACGAATTTGCTGGGGAA
TCTCGTTTTTCTTCTTAAGACTTTTGGGACATGGTTTGACTCCCGAACATCACCGACGCGTC
TCCTGCTG 3'
a) 5' TTCCGGAAGAAGCTTATACGG 3'
b) 5' CTGTGTTCACCTAATATTCCT 3'
c) 5' CAGCAGGAGACGCGTCGGTGA 3'
d) 5' AGGAATATTAGTATAATCCAC 3'
e) 5' GACGCGTCGGTGATGTTCGGG 3’
f) 5' ATCTCTATTTAATATTTATGT 3'
Answer (word limit 250 words max):
Question 2 (10 marks, 250 words max) Discuss factors that might influence the
activity of DNA-binding proteins (transcription factors) and bring about changes in
gene expression (8 marks). Include in your answer
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