What size would the PCR product be for a different chromosome where there were nine tandem repeats.

Q: What is the name of the DNA duplication process? What is the main enzyme that participates in it?

A: Every living cell divides to form multiple cells. During this division process, there is another…

Q: Outline the major steps invalved in recombinant DNA technalogy required to clane a gene into a…

A: INTRODUCTION Recombinant DNA technology In recombinant DNA technology we insert our gene of interest…

Q: Is the given statement that the first step of PCR process involves heating of DNA solution TRUE or…

A: The reaction that is used to make the duplicates of a particular DNA segment is known as polymerase…

Q: What would be the final primer concentration if 0.5 ul of 10 uM primers were ad a PCR reaction with…

A: Introduction: A primer is a short strand of DNA or RNA that is usually about 18-22 bases and serves…

Q: why different thermal conditions are necessary for successful PCR?

A: DNA polymerase is an enzyme that naturally polymerizes nucleotides to polynucleotide strands. The…

Q: Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain…

A: Polymerase (PCR) chain reaction is a process, during which the specific DNA segment is copied in a…

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: Why is MgCl2 added to the PCR reaction?

A: De-novo gene synthesis is becoming an important tool for recombinant DNA technology. The Denovo gene…

Q: Why Sanger Sequencing Dependson DNA Polymerase?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR…

A: Standard PCR reagents include a set of appropriate primers for the desired target gene or DNA…

Q: Is the given statement that the first step of PCR process involves heating of DNA solution TRUE or…

A: PCR process is a straightforward however valuable methodology in molecular science called the…

Q: DNA hybridization process using probe

A: DNA Hybridization is the process of combining two complementary DNA single strands allowing them to…

Q: What is recombination? Mention its applications with reference to genetic engineering.

A: Answer: Introduction: Recombination means the method of generating a new blend of genes by crossing…

Q: What is the role of NaCl in the DNA isolation procedure?

A: NaCl (Sodium Chloride) is used during various DNA isolation procedures. Usually, in DNA isolation,…

Q: what kinds of techniques are used to sequence large fragments of dna upto 50kb

A: With the advancement of science and technology in the field of life sciences, there has emerged…

Q: Why is extra primers added to the pcr reaction?

A: Introduction :- The polymerase chain reaction, or PCR, is a chemical reaction used by molecular…

Q: 4b) Chargaff was the first person to isolate large strands of genomic DNA, rather than small…

A: DNA is the hereditary material in humans and almost all other organisms. Nearly every cell in a…

Q: Why were both “+ DNA” and “- DNA” mixtures prepared and plated?

A: Both “+DNA” and “-DNA” mixtures prepared and plated because in the transformation protocol “+DNA”…

Q: 1b: Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer.…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: What is the function of the chaotropic salt in DNA isolation?

A: These salts are strong protein denaturing agents.

Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?

A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…

Q: What is the role of EDTA in the DNA isolation procedure?

A: DNA isolation is a laboratory technique used to obtain purified DNA from various samples, including…

Q: How did Messelson and Weigle demonstrate recombination?

A: In 1961, Messelson and Weigle have experimented with the recombination processes in the lambda…

Q: Determine which primer pair is the best choice. • Explain why the other primers are not good…

A: A brief DNA or RNA sequence serves as the template and controls the production of a complementary…

Q: Component 1 PCR buffer 2 MgCl2 3 dNTP mix 4 SSR forward primer 5 SSR reverse primer 6 Taq polymerase…

A: PCR is also known as a polymerase chain reaction which is generally used to amplify the template DNA…

Q: Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the…

A: DNA is extracted from various sources to study the sequence of the DNAs from various sources and to…

Q: What is a restriction digest? What does it mean if you were given a precut DNA?

A: Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at…

Q: If the PCR negative was positive what would that mean?   If the PCR positive was negative what…

A: PCR is also known as polymerase chain reaction is used to amplify the DNA and get accurate results…

Q: Nikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a…

A: It is possible to amplify a particular DNA fragment using the molecular biology technique known as…

Q: What special kind of nucleotide is used in the Sanger sequencing procedure?

A: All life is dependent on DNA. It contributes to the genetic code for life and its activities,…

Q: Prepare an 35 µL PCR reaction mixture and set thermal conditions for gene amplification?

A: PCR or the polymerization chain reaction is the molecular biology technique used to make multiple…

Q: In the third step of the PCR, the temperature is raised to 73 degrees C.  What happens during this…

A: PCR: PCR (polymerase chain reaction) is a technique used to make multiple copies of a particular…