2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the number cycles of PCR required to generate 1 ng of DNA, when starting from a single copy. Note that the molar mass of an average DNA bp is 600 g/mol.

Q: You are using a spectrophotometer to determine the concentration of DNA in a PCR product, but your…

A: The concentration of DNA and RNA should be determined by measuring the absorbance at 260 nm (A260)…

Q: 5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template…

A: A primer is a short strand of nucleotide molecules that serves as an initial point for the synthesis…

Q: If you design a set of primers for a PCR reaction, please describe the temperature that you will set…

A: PCR This is Polymerase Chain Reaction. This technique is used to amplify the specific sequences of…

Q: Calculate the volume (in µL) of DNA template required to add 60.0 ng to a 20.0 µL PCR if the DNA…

A: Concentration is the term used to define the amount of a specific entity with respect to the total…

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: Explain why a quantitative PCR analysis cannot determine the size of the initial template sequence…

A: The combination of the probe-based and intercalating dye-based methods brings the potential…

Q: What size would the PCR product be for a different chromosome where there were nine tandem repeats.

A: The each STR is 5 bp. So, in the given diagram the total length of the PCR product is 420 bp. It…

Q: What would be the expected effect on the PCR reaction, if you increased the temperature of the…

A: The polymerase chain reaction (PCR) is a technique for amplifying DNA sequences in the laboratory.…

Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…

A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…

Q: The process of PCR essentially revolves around three phases.  Briefly describe these phases and the…

A: The polymerase chain reaction (PCR) is a widely used technique in molecular biology that allows…

Q: In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA…

A: Here, option A is the right answer. You may use the following formula to calculate the number of…

Q: A student is trying to add 15.0 ng of DNA template to a 20.0 µL PCR.  The DNA template is at a…

A: Polymerase Chain Reaction or PCR is a method to make multiple copies of DNA starting from a small…

Q: List essential components required for the DNA sequencing reaction based on the Sanger method.…

A: DNA sequencing is the process of determining the sequence of nucleotides  in a piece of DNA. In…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

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Q: What allows the peaks to be different colors?

A: DNA sequencing refers to the determination of the nucleotide sequence in the DNA molecule. Automated…

Q: If a sample of double-stranded DNA gave the following results, what is the concentration (ng/uL) of…

A: The absorption value of 1 at A260 corresponds to 50 ug/ml So, the value of 1.637 at A260 corresponds…

Q: List and briefly describe the function of 4 proteins used in DNA replication in a E. coli but NOT…

A: The process of copying a DNA molecule to produce its two identical copies is termed as the DNA…

Q: Lane 1 = TakaraBio 200 bp DNA ladder Lane 2 = Haell only; (thick band) 1883 bp and 1871 bp, 799 bp…

A: Understanding the restriction digestion patterns of any plasmid is very important to know where…

Q: You Purified plasmid DNA (a 6.0 kb vector) and got the following results... DNA conc: 200 ng/µL…

A: Plasmid DNA is mainly a small, circular, double stranded DNA molecule and also it is different from…

Q: Tell me how did you prepared the gel if the tray is 150 ml? how many milliliters of the TBE and how…

A: The answer to the first question is, 3 grams of agarose in 150 ml of TBE.   *to get the second…

Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

A: Dilution is defined as the ratio of volume from stock to the final volume.  Dilution= Stock volume /…

Q: Each PCR cycle has three steps: DNA sample denaturation, primer annealing, and elongation/extension…

A: Polymerase chain reaction (PCR) is a simple technique to amplify a DNA template to produce specific…

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A:

Q: During PCR, the reaction mixture cycles through three temperatures (for example 94, 60, and 72…

A: Introduction :  Using the thermo-resistant DNA polymerase, the polymerase chain reaction, also known…

Q: Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR…

A: 5) A. Amount of purified PCR product DNA in the tube = 275 ng = 275 000 pg (1000 pg = 1 ng) The…

Q: Component 1 PCR buffer 2 MgCl2 3 dNTP mix 4 SSR forward primer 5 SSR reverse primer 6 Taq polymerase…

A: PCR is also known as a polymerase chain reaction which is generally used to amplify the template DNA…

Q: For your PCR reaction above, the primer set has a much higher AT (low GC) content than what wo be…

A: The content you provided discusses the concept of annealing temperature in the context of Polymerase…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…

Q: Summarize the key concept of biology

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Q: How many microliters of pGLO plasmid will you need for a PCR reaction is you need 200 nanograms and…

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Q: In PCR amplification Why is it important to know the length of the sequence you amplify?

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Q: Explain why a positive control and negative control are included in PCR experiments. ​Explain the…

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Q: All of the following are performed during restriction fragment length polymorphism analysis. 1.…

A: DNA( Deoxyribonucleic acid) is basically a helical, twisted structure which comprises of two…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

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Q: The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis…

A: PCR is a process in which millions of copies of a specific sequence of DNA can be made in a matter…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: The polymerase chain reaction is abbreviated as PCR. It is a molecular biology technique for…

Q: please solve this with step-by-step calculations and explanations.

A: Let's break this problem down step by step. Part (a) Setting up the PCR reactionsFor each PCR…

Q: 1) Describe the ingredients of a PCR reaction.  List different temperatures used in each cycle and…

A: Answer:- Template DNA, PCR primers - a starting nucleotides sequence pre-requisite for the…

Q: You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many…

A: DNA is a double-stranded helix where two strands of DNA are linked to each other via hydrogen…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.