You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase (5 units/ul), and each PCR reaction requires a final of 10 units of Taq polymerase. You have a microfuge tube of Taq Buffer (5X), and each PCR reaction requires a final of 1X Taq Buffer. How much of Taq polymerase and Taq buffer would you add? Select all that apply 10 ul of Taq buffer 2 ul of Taq polymerase 5 ul of Taq buffer 5 ul of Taq polymerase

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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You want to set up 50 µl total volume of a PCR reaction.
You have a microfuge tube of Taq polymerase (5 units/ul), and each PCR reaction
requires a final of 10 units of Taq polymerase.
You have a microfuge tube of Taq Buffer (5X), and each PCR reaction requires a final of
1X Taq Buffer.
How much of Taq polymerase and Taq buffer would you add? Select all that apply
10 ul of Taq buffer
2 ul of Taq polymerase
5 ul of Taq buffer
5 ul of Taq polymerase
Transcribed Image Text:You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase (5 units/ul), and each PCR reaction requires a final of 10 units of Taq polymerase. You have a microfuge tube of Taq Buffer (5X), and each PCR reaction requires a final of 1X Taq Buffer. How much of Taq polymerase and Taq buffer would you add? Select all that apply 10 ul of Taq buffer 2 ul of Taq polymerase 5 ul of Taq buffer 5 ul of Taq polymerase
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