Make the PCR Cocktail I field out my work.. but I don't know which one is incoeert.. This table lists the ingredients, stock reagent concentrations, and concentrations in the PCR reaction. Prepare a "PCR cocktail" to be added to your samples to achieve these concentrations. Make enough cocktail to run nine samples. [Four student samples + three positive controls + one negative control + one extra.] {Hint: Remember that the concentration in the reaction is not the same as the concentration in the cocktail!] Component Stock Concentration Concentration in the PCR reaction Volume per reaction Volume to make cocktail Sterile water - - 0µl 0µl PCR buffer w/ MgCl2 10x 1x 4µl 36µl Nucleotide mix 10 mM 0.2 mM 0.8µl 7.2µl Primer 1 (Forward) 10 µM 1.0 µM 4µl 36µl Primer 2 (Reverse) 10 µM 1.0 µM 4µl 36µl Taq DNA polymerase 5 U/µl 1.0 U 8µl 72µl DNA template (sample) - ~1 ng 20 µl 180µl Total - - 40 µl 360 µl

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