In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: A next-generation method of DNA sequencing, 454 sequencing, uses pyrosequencing in which the…

A: Pyrosequencing is a next-generation technique of DNA sequencing. This method can be used to detect…

Q: The role of hemoglobin in transport of oxygen and carbon dioxide in the lung capillaries and in…

A: Hemoglobin is a protein found in red blood cells that plays a crucial role in the transport of…

Q: You have 10 ul of DNA you want to cut with HindIII, and then run a gel, in order to cut out certain…

A: Restriction Digestion is a process by which DNA molecules are cut into small pieces with the aid of…

Q: In Figure 10-14, why does DNA migrate to the anode(+ pole)?

A: Deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: In an experiment a student’s sample amplified for D1S80 produced 3 bands. It was the only DNA sample…

A: PCR  amplified sample is run on gel electrophoresis for the detection of  results and  comparing it…

Q: Why is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any…

A: DNA can be extracted from any tissue or cell. There are four basic steps of DNA extraction. They…

Q: Describe the processes involved in denaturing and renaturing of DNA, and explain what is useful…

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…

Q: What was the function of each solution or step in the DNA purification protocol? The following…

A: Deoxyribonucleic acid extraction is the isolation and purification of DNA from sample using a…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: What is the role of NaCl in the DNA isolation procedure?

A: NaCl (Sodium Chloride) is used during various DNA isolation procedures. Usually, in DNA isolation,…

Q: What variables can affect the transformation efficiency?

A: Introduction  The ability of cells to absorb extracellular DNA and express the genes that are…

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: DNase is an endonuclease that degrades DNA. Which steps or/and reagents in the DNA isolation…

A: Restriction enzymes can be divided into two types viz., restriction endonuclease and restriction…

Q: A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2%…

A: During agarose gel electrophoresis, an agarose solution is prepared, it is cooled to prepare a gel,…

Q: What is the source of agarose, the powder used to make the gel?                        What is the…

A: Gel electrophoresis is a technique used by scientists to separate DNA or protein molecules.  Gel…

Q: For PCR, what property of DNA does temperature influence? Why do the “denaturation” and “annealing”…

A: PCR stands for polymerase chain reaction. It is an in-vitro laboratory technique used for generating…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: Why were both “+ DNA” and “- DNA” mixtures prepared and plated?

A: Both “+DNA” and “-DNA” mixtures prepared and plated because in the transformation protocol “+DNA”…

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples…

A: Gel electrophoresis is a process that helps in the separation of DNA fragments based on sizes with…

Q: You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the…

A: Agarose Gel Electrophoresis (AGE), is an biochemistry technique which is used to estimate DNA…

Q: What is the role of EDTA in the DNA isolation procedure?

A: DNA isolation is a laboratory technique used to obtain purified DNA from various samples, including…

Q: To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on…

A: Specific sequences can be amplified by PCR in order to generate large amounts of DNA to manufacture…

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the…

A: Insulin is a natural peptide hormone produced by the beta cells of the pancreas in response to high…

Q: The genome of a typical bacterium contains about 5 x 106 base pairs, and can be replicated in about…

A: Replication is the process of synthesis new daughter DNA strand using the existing parental DNA…