What variables can affect the transformation efficiency?

Q: Calculate the volume (in µL) of DNA template required to add 60.0 ng to a 20.0 µL PCR if the DNA…

A: Concentration is the term used to define the amount of a specific entity with respect to the total…

Q: Which of the following plates could represent the results of the transformation experiment with PGLO…

A: INTRODUCTION Transformation is a genetic exchange mechanism by which “naked” DNA is taken up by…

Q: What is a poor transformation efficiency?

A: Transformation is a natural phenomenon mostly observe in many bacteria. It is dependent on the…

Q: A next-generation method of DNA sequencing, 454 sequencing, uses pyrosequencing in which the…

A: Pyrosequencing is a next-generation technique of DNA sequencing. This method can be used to detect…

Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?

A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…

Q: A student is trying to add 15.0 ng of DNA template to a 20.0 µL PCR.  The DNA template is at a…

A: Polymerase Chain Reaction or PCR is a method to make multiple copies of DNA starting from a small…

Q: List essential components required for the DNA sequencing reaction based on the Sanger method.…

A: DNA sequencing is the process of determining the sequence of nucleotides  in a piece of DNA. In…

Q: Why do we need to determine the extinction coefficient in order to calculate the initial velocity in…

A: Enzyme are proteins that catalyse biochemical reactions. A substrate binds at the active site of the…

Q: You now redo the experiment but instead of an SDS gel you run a native gel followed by completing…

A: Western blotting is the technique of identification of a specific protein in a given sample.

Q: In sequencing, dideoxyribonucleotides (ddNTP) are used that terminate the amplification. Why do they…

A: Introduction : DNA sequencing is the process by which the specific sequence of the four nucleotide…

Q: What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…

Q: What is transformation efficiency and how is it calculated?

A: Transformants are the cells that are able to incorporate any modified foreign or artificial DNA.…

Q: Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration…

A: An assay is a biochemical lab procedure to determine the quantity of molecules of interest in a…

Q: Why is the model with induced fit more probable than the one with lock and key?

A: Any of a number of proteins produced in living cells that speed up or catalyze an organism's…

Q: What is the formula for transformation efficiency?

A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples…

A: Gel electrophoresis is a process that helps in the separation of DNA fragments based on sizes with…

Q: What are the two main mechanisms by which cells repair doublestrandbreaks? Briefly describe each…

A: The DNA (deoxyribonucleic acid) is the hereditary nucleic acid sequence that contains the genetic…

Q: why are 2D NMR spectra symmetrical

A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…

Q: Which parameters are plotted on the X and Y axes of a Kaplan-Meier graph?

A: During scientific experiments, a huge amount of data is generated. Different types of experimental…

Q: CPT code assignment 44970

A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…