What variables can affect the transformation efficiency?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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What variables can affect the transformation efficiency?

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The ability of cells to absorb extracellular DNA and express the genes that are encoded by it is known as transformation efficiency. This is dependent on the cells' competence. It can be estimated by dividing the quantity of DNA utilised during a transformation technique by the number of successful transformants. Transformants are cells that have ingested DNA that is foreign, synthetic, or altered and that can express genes on the added DNA.

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