Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
What is the formula for transformation efficiency?
Transformation:
Transformation is a process of delivering a genetic element inside a bacterial cell. The transformation of this term is generally applied for bacteria or prokaryotes. Transformation does not require a living donor cell.
Transformation efficiency:
It is efficiency through which cells can take extracellular DNA and show genes encoded by it. It is mainly based on the competence of the cells. It is an important tool in molecular biologists, microbiologists. Transformation efficiency is expressed as colony-forming units per microgram of DNA and the transformation efficiency can tell something about how competent the cells were, in other words, how capable of taking up the DNA they were and the quality of the DNA.
The formula for transformation efficiency or it is calculated is the number of colonies forming units per microgram of DNA.
Transformation efficiency formula
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