11. Calculate K and Vmax from the following data: M [S] (M) (mM.s¹) 0.1 0.34 0.53 0.74 0.91 1.04 0.2 0.4 0.8 1.6
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![11. Calculate K and Vmax from the following data:
M
[S] (μM) v. (mM.s¯¹)
0.1
0.34
0.2
0.53
0.4
0.74
0.8
0.91
1.6
1.04
12. Explain why each of the following data sets from a Lineweaver-Burk plot are not individually ideal for determin
Set A 1/[S] (mM-¹) 1/2. (µM-](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4ddf1583-af9c-4cdb-b832-89f0a6e45ec4%2F38b30ed3-b126-4868-80eb-7de2978f6909%2Fjhr74ax_processed.jpeg&w=3840&q=75)
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- A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.Two compounds with partition coefficients of 15 and 18 areto be separated on a column with Vm/Vs 3.0 and tm 1.0 min.Calculate the number of theoretical plates needed to produce aresolution of 1.5.
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