How we can select Tm value for the primers? Please answer at your own words.
DNA replication is considered a biological process during cell division, where DNA can make a copy of itself and results in the formation of two identical DNA. During replication, the double helix DNA gets separated at a specific point by breaking the hydrogen bonds between the complementary strands. It is followed by DNA synthesis initiated by a short nucleic acid sequence called primers.
The polymerase chain reaction(PCR) is considered a molecular technique that implies DNA replication on a grander scale. In the PCR process, the temperature required to break the double-stranded DNA into single-standard DNA is termed the temperature of melting(Tm). The optimal temperature of melting for the DNA ranges from 50°C and 100°C.
Step by step
Solved in 2 steps
