Will glycolysis be useful for the metabolism of acetate? (Yes or No)  2. When grown aerobically, what is the highest number of NADH molecules that can be generated?  3. When grown aerobically, what is the highest number of FADH2 molecules that can be generated?  4. When grown aerobically, what is the highest net ATP (output-input, including transport) that can be generated?  5. When grown anaerobically by fermentation, what is the highest net ATP (output-input, including transport) that can be generated?  6. Can this organism survive by fermentation of acetate alone? (Yes or No)

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Please answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24.

As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain.

Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. 

Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an answer of 0.

1. Will glycolysis be useful for the metabolism of acetate? (Yes or No) 

2. When grown aerobically, what is the highest number of NADH molecules that can be generated? 

3. When grown aerobically, what is the highest number of FADH2 molecules that can be generated? 

4. When grown aerobically, what is the highest net ATP (output-input, including transport) that can be generated? 

5. When grown anaerobically by fermentation, what is the highest net ATP (output-input, including transport) that can be generated? 

6. Can this organism survive by fermentation of acetate alone? (Yes or No) 

 

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