What order should the steps be in for this culture method?

Q: If a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it…

A: "Microbiology" is the study of microorganisms and associated topics. Microbiology has gone a long…

Q: Why is the plate count method a determination of the number of viable cells?

A: The plate count method, also referred to as the viable count method or colony-forming unit (CFU)…

Q: List and explain the Sterile Technique What's the difference between Medical vs. Surgical Asepsis?…

A: Aseptic technique or Sterile technique means using practices and procedures to prevent contamination…

Q: What are the four steps of an Elisa protocol

A: Enzyme-linked immunosorbent assay (ELISA)  is an immunological assay commonly used to measure…

Q: In a COVID test, why is it necessary to swab all the way at the back of the nose and throat

A: The COVID-19 pandemic has currently affected the whole world and has claimed a huge number of lives.…

Q: What salt did Meselson and Stahl use for their ultracentrifuge gradients? O NaCI O NH4CI O CSCI O…

A: Given: DNA replicates semiconservatively. Matthew messelson and Franklin stahl performed an…

Q: During the Lederberg and Tatum experiment, theresearchers compared the growth of mutant strains…

A: Conjugation is the process by which bacteria transfers its genetic material to the other bacteria by…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: Give three situations where negative staing procedure would be used?

A: Negative staining is a staining technique in which the background is stained whereas the specimen…

Q: ELISAs require a number of steps to be completed in order for the test to work. Why do you need to…

A: Enzyme-linked immunosorbent assay is commonly known as ELISA where Ag-Ab interaction is monitored by…

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: o What is a pure culture? o Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: What is the procedure of beta method?

A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…

Q: State the four steps of Microarray Anaylsis.

A: Using microarray analysis, it is possible to assess gene expression levels as well as recognise…

Q: Why is a positive and a negative control used in a Elisa experiment?

A: The enzyme-linked immunosorbent assay (ELISA) is a laboratory technique for detecting and…

Q: a) Make a drawing of the cells from each culture to identify the various stages of the cell…

A: Saccharomyces cerevisiae is a eukaryotic organism that is used in various scientific experiments. It…

Q: ow do you figure out a number of live cells using flow cytometry results?

A: Flow Cytometry is a technique used to determine and analyze the characteristics of cells using…

Q: What are the factors which need to be considered in the selection of culture vessel?

A: Culture vessels:- Culture vessels provide a contamination barrier to protect the cultures from the…

Q: What is the purpose of staining bacteria? What is the purpose of collecting bacteria from different…

A: The study regarding unidentified bacteria is explained to the reader in this part, along with its…

Q: In bealdle and tatums experiments the immediate effect of the X-ray treatments was ?

A: Enzymes are the proteins that catalyze various metabolic reactions. Proteins are formed as a result…

Q: Explain the mechanisms of the three types of ELISA we discussed in lab: 1) direct, 2) indirect, 3)…

A: ELISA known as enzyme linked immunosorbent assay. This technique is commonly used for the detection…

Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: What is the purpose of the blank test in the experiments?

A: Examples of different types of blanks used in different experiments are - equipment blank, field…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.