Write the process of, building a recombinant plasmid? Or how you can build a recombinant plasmid?

Q: In the genotype presented (genomic plus plasmid genes), which of the following statements Is…

A: Lac operon codes Lac Z,Y,A genes which are structural genes encoding Beta galactosidase,Permease,…

Q: Provide 2 diagrams to illustrate the 2 potential recombinant plasmids,

A: The question here states about the concerned recombination of cloned plasmids and their…

Q: pXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can…

A: Plasmids are the extra chromosomal material which are present independently of  the main…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the  plasmid…

A: Deoxyribonucleic acid ( DNA) is the molecule that carries genetic information for an organism.The…

Q: Why is the presence of insertion sequences on the F plasmid significant? What are F- prime (or F')…

A: The process of fusion of two compatible bacterial cells, is known as Conjugation. It requires the…

Q: What does the F plasmid code for?

A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…

Q: Using a diagram, and 3-5 sentences, explain the relationship between cell immortality and telomerase…

A: Each time a cell divides, the telomeres get shorter. Telomeres are a region of repetitive nucleotide…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer…

A: Agrobacterium tumefaciens is a soil bacterium that naturally infects plants and produces tumors by…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction mapping involves physically cuttings double stranded DNA by using restriction…

Q: A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I:…

A: Restriction endonucleases are enzymes that cut DNA at specific sites. Restriction endonucleases…

Q: Briefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at…

A: DNA is a type of nucleic acid that is available in the nucleus of the cell. It is the chemical…

Q: Many studies have linked plasmids to bacterial resistance to antibiotics.  Closured circular…

A: Focusing on plasmids as a target to combat antibiotic resistance holds promise but requires a…

Q: Why is it easy to transform a bacterial cell and then use that to transform an animal or plant cell?…

A: In molecular biology, transformation is the process where the genetic alteration of a cell is…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Plasmids are circular double-stranded DNA elements. These are found in prokaryotes. The plasmids…

Q: EDTA weakens the cell wall by removing ions that help hold it together while glucose prevents…

A: EDTA disrupts the outer membrane (enzymes that degrade DNA) by inhibiting DNases and chelating…

Q: You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a…

A: Other incorrect options:B. This technique involves introducing the ice gene directly into the T-DNA…

Q: There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA…

A: The cellular functions are regulated/controlled by the DNA present within the nucleus of the cell.…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Removing RNA is one of the crucial processes in plasmid purification, especially after the manual…