Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer virulence Amp selectable marker 1. Genes essential for the transfer of DNA from A tumefaciens to a plant cell are contained in the region of the plasmid 2. Genes that enable A. tumefaciens to conjugate with a plant cell are in the the plasmid region of 3. The gene that enables scientists to identify A. tumefaciens bacteria that have acquired the disarmed plasmid is the 4. The gene that enables scientists to identify A. tumefaciens bacteria that have acquired the transformation vector is the 5. The gene of interest that scientists want to insert into the plant genome to alter its phenotype is located in the of the transformation vector 6. The gene that enables scientists to identify plant cells that have acquired the DNA from the T-region of the transformation vector is the
Kan selectable marker plant selectable marker constitutive promoter T-region conjugative transfer virulence Amp selectable marker 1. Genes essential for the transfer of DNA from A tumefaciens to a plant cell are contained in the region of the plasmid 2. Genes that enable A. tumefaciens to conjugate with a plant cell are in the the plasmid region of 3. The gene that enables scientists to identify A. tumefaciens bacteria that have acquired the disarmed plasmid is the 4. The gene that enables scientists to identify A. tumefaciens bacteria that have acquired the transformation vector is the 5. The gene of interest that scientists want to insert into the plant genome to alter its phenotype is located in the of the transformation vector 6. The gene that enables scientists to identify plant cells that have acquired the DNA from the T-region of the transformation vector is the
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:Scientists modified the tumor-inducing (Ti) plasmid, a naturally occurring plant plasmid found in Agrobacterium tumefaciens, to create a tool used to introduce any gene of interest into plant cells. They created a binary system because a single Ti plasmid is too large to be easily manipulated. One part of the system is a disarmed
plasmid, and the second part is a transformation vector. The following sentences describe the function of key DNA elements in the system.
Virulence
region
Genes for
conjugative transfer
Disarmed
Ti plasmid
(T-region removed)
ori
Kan selectable marker
plant selectable marker
constitutive promoter
T-region
conjugative transfer
virulence
Kan
(bacterial
selectable
marker)
Amp selectable marker
Plant selectable marker
(e.g., herbicide resistance)
Constitutive
promoter
T-DNA left
border
Place the terms in the appropriate blanks to complete the sentences. Not all terms will be used.
3' transcriptional terminator
MCS (inserted
gene of interest)
T-region
Transformation
vector
ori
T-DNA right border
Amp
(bacterial
selectable
marker)
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. Genes essential for the transfer of DNA from A. tumefaciens to a plant cell are contained in the
region of the plasmid.
2. Genes that enable A. tumefaciens to conjugate with a plant cell are in the
the plasmid.
region of
3. The gene that enables scientists to identify A. tumefaciens bacteria that have acquired the disarmed
plasmid is the
4. The gene that enables scientists to identify A. tumefaciens bacteria that have acquired the
transformation vector is the
5. The gene of interest that scientists want to insert into the plant genome to alter its phenotype is
located in the
of the transformation vector.
6. The gene that enables scientists to identify plant cells that have acquired the DNA from the T-region
of the transformation vector is the
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