Homogenize cells Centrifuge Heat-kill Recover IIIS filtrate IIIS cells spun to bottom of tube Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with Treat with ribonuclease Treat with deoxyribonuclease protease Assay for Transformation IIR cells IIR cells IIR cells IIR cells + + IIIS filtrate DNase-treated IIIS filtrate RNase-treated IIIS filtrate Protease-treated IIIS filtrate No transformation Transformation Transformation Transformation occurs occurs occurs occurs

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Homogenize
cells
Centrifuge
Heat-kill
Recover IIIS filtrate
IIIS cells spun to
bottom of tube
Extract carbohydrates,
lipids, and proteins
IIIS cells in liquid
culture medium
Treat with
ribonuclease
Treat with
Treat with
deoxyribonuclease
protease
Assay for
Transformation
IIR cells
IIR cells
IIR cells
IIR cells
+
IIIS filtrate
DNase-treated
RNase-treated
IIIS filtrate
Protease-treated
IIIS filtrate
IIIS filtrate
No transformation
Transformation
Transformation
Transformation
occurs
occurs
occurs
occurs
IIR cells
IIR cells
IIR cells
Only IIR
cells
+
IIIS cells
IIIS cells
IIIS cells
Conclusion:
Active factor is DNA
Conclusion:
Active factor is not RNA
Conclusion:
Active factor is
Control:
IIIS contains
not protein
active factor
E IIIS cells
EA IIR cells
8. For each molecule listed, indicate whether they caused a transformation (Yes)
or did not cause a transformation (No):
a. RNA
b. DNA
c. Proteins yes
d. Carbohydrate
e. Lipids
Transcribed Image Text:Homogenize cells Centrifuge Heat-kill Recover IIIS filtrate IIIS cells spun to bottom of tube Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with ribonuclease Treat with Treat with deoxyribonuclease protease Assay for Transformation IIR cells IIR cells IIR cells IIR cells + IIIS filtrate DNase-treated RNase-treated IIIS filtrate Protease-treated IIIS filtrate IIIS filtrate No transformation Transformation Transformation Transformation occurs occurs occurs occurs IIR cells IIR cells IIR cells Only IIR cells + IIIS cells IIIS cells IIIS cells Conclusion: Active factor is DNA Conclusion: Active factor is not RNA Conclusion: Active factor is Control: IIIS contains not protein active factor E IIIS cells EA IIR cells 8. For each molecule listed, indicate whether they caused a transformation (Yes) or did not cause a transformation (No): a. RNA b. DNA c. Proteins yes d. Carbohydrate e. Lipids
Expert Solution
Step 1

Transformation is the process by which microbes uptake genetic material from the surroundings and express that genetic material.

When a non- virulent strain of bacteria uptakes DNA of a virulent bacteria from the surrounding it transforma into a virulent bacteria. 

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