Graph SEQ Graph ARABIC I p24 po/ml 10 3) Quantitative skills: Using the graph below, determine the approximate concentration of p24 capsid protein (in kg/L) after 6 days of transfection with the 168.1/R molecular clone. 168.10 10 Include your calculations within the following space: 108.1 18.VT 10 1.V0 10 4 6 days after transfection 10 12 14

Graph SEQ Graph ARABIC I p24 po/ml 10 3) Quantitative skills: Using the graph below, determine the approximate concentration of p24 capsid protein (in kg/L) after 6 days of transfection with the 168.1/R molecular clone. 168.10 10 Include your calculations within the following space: 108.1 18.VT 10 1.V0 10 4 6 days after transfection 10 12 14

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 33SST

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Considering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)
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וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…
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AGAROSE GEL ELECTROPHORESIS PRELAB ANSWER ALL QUESTIONS 1. Type of nucleotide is one of the factors that influence electrophoretic mobility. a) True b) False 2. Electrophoresis is used for DNA separation and not proteins. a) True b) False 3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis. a)True b)False 4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator. a) True b) False 5. During electrophoresis, DNA fragments collect at the Cathode. a) True b) False
How can you calculate the final concentrations of these when final volume is 50 µl? • 2 µl of 5 ng DNA template (α2 cDNA in pAWneo2 vector, stock concentration 2.5 ng/µl) • 1 µl of dNTP mix (10 mM dATP, 10 mM dTTP, 10 mM dCTP and 10 mM dGTP) • 2 µl of α2I forward primer (5 µM) • 2 µl of α2I reverse primer (5 µM) • 2.5 µl of OneTaq polymerase (0.5 U/µl)