You will be given the plaque assay plates to analyse from three different bacteriophages stocks You need to determine which bacteriophage should be used in subsequent transductions. You should give a reason why. PHYSIOLOGY AN GENETICS I Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.

You will be given the plaque assay plates to analyse from three different bacteriophages stocks You need to determine which bacteriophage should be used in subsequent transductions. You should give a reason why. PHYSIOLOGY AN GENETICS I Experimental set up: all culture plates were incubated at 37°C over night Bacteriophage A 200 μl of bacteriophage A was added to 800 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-4. 100 μl of the mixed culture was then added to 9.9 ml molten agar and poured into the petri dish. Bacteriophage B 100 μl of bacteriophage B was added to 900 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-5. 1000 ul of the mixed culture was then added to 9 ml molten agar and poured into the petri dish. Bacteriophage C 250 μl of bacteriophage C was added to 750 μl of E. coli broth culture. The mixed culture was then diluted 1/10 to 10-6. 1000 μl of the mixed culture was then added to 9 ml molten agar and poured into the petri dish.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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